Brain delivery of intranasal in situ gel of nanoparticulated polymeric carriers containing antidepressant drug: behavioral and biochemical assessment
Brain delivery of intranasal in situ gel of nanoparticulated polymeric carriers containing antidepressant drug: behavioral and biochemical assessment
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DOI：
10.3109/1061186x.2014.994097
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发表时间：
2015-04-01
影响因子：
4.5
通讯作者：
Goyal, Amit K.
中科院分区：
文献类型：
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作者：
Kaur, Prabhjot;Garg, Tarun;Goyal, Amit K.
本研究旨在鼻内给药后盐酸曲马多（中枢作用合成阿片样物质）的脑递送用于治疗抑郁症。通过离子凝胶化方法制备壳聚糖纳米颗粒（NPs），然后将开发的NPs与基于Pluronic和HPMC的粘膜粘附热可逆凝胶一起添加。根据粒径、包封率、体外释放度等参数优化处方。采用强迫游泳实验诱导抑郁，并通过各种行为学和生化指标进行评价。此外，结果显示，与对照组相比，自发活动、体重显著增加。还显示了生物化学参数的改变，如谷胱甘肽水平和过氧化氢酶水平显著增加，而脂质过氧化和亚硝酸盐水平在鼻内给予制剂后降低。因此，鼻内TRM HCl NP-负载原位凝胶被发现是用于治疗抑郁症的有前途的制剂。
This study was aimed for brain delivery of Tramadol HCl (centrally acting synthetic opioid) following intranasal administration for treatment of depression. Chitosan nanoparticles (NPs) were prepared by ionic gelation method followed by the addition of developed NPs with in the Pluronic and HPMC-based mucoadhesive thermo-reversible gel. Developed formulation optimized based on the various parameters such as particle size, entrapment efficiency, in vitro release study. Depression induction was done by forced swim test and evaluated by various behavioral and biochemical parameters. Furthermore, results showed significantly increased in locomotors activity, body weight as compared to control group. It also showed alteration in biochemical parameters such glutathione level and catalase levels significantly increased other than lipid peroxidation and nitrite level was found to be decreased after intranasal administration of formulation. Thus, intranasal TRM HCl NP-loaded in situ gel was found to be a promising formulation for the treatment of depression.