Vestigial suppresses apoptosis and cell migration in a manner dependent on the level of JNK-Caspase signaling in the Drosophila wing disc
Vestigial suppresses apoptosis and cell migration in a manner dependent on the level of JNK-Caspase signaling in the Drosophila wing disc
复制标题
Vestigial 以依赖于果蝇翼盘中 JNK/Caspase 信号传导水平的方式抑制细胞凋亡和细胞迁移
DOI：
10.1111/1744-7917.12762
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发表时间：
2020-04-13
期刊：
影响因子：
4
通讯作者：
Shen, Jie
中科院分区：
文献类型：
--
作者：
Fan, Wen-Min;Luo, Dan;Shen, Jie
Dpp和Wg信号通路在果蝇的许多生物学过程中起着重要的作用。果蝇退化基因（vg）是翅成虫盘细胞增殖所必需的，是成虫翅和笼头结构形成所必需的，并受Dpp和Wg信号的调控。使用果蝇侵袭模型的翅膀上皮，我们在此表明，抑制Dpp或Wg信号促进细胞迁移跨越细胞谱系限制性前/后（A/P）室边界。作为Dpp和Wg信号传导的下游，vg可以阻断由任一途径的丧失诱导的细胞迁移。此外，vg的抑制足以诱导细胞迁移穿过A/P边界。转录组学分析揭示了在抑制翼盘中的vg后参与细胞迁移的潜在下游基因。我们进一步证明c-Jun N-末端激酶（JNK）信号通过上调Caspase活性促进由vg抑制诱导的细胞迁移。总之，我们的研究结果揭示了Vg抑制细胞迁移的要求，并阐明了发育信号如何合作，以稳定细胞沿着室边界。
The Decapentaplegic (Dpp) and Wingless (Wg) signal pathways play important roles in numerous biological processes in Drosophila. The Drosophila vestigial (vg) gene is selectively required for wing imaginal disc cell proliferation, which is essential for the formation of the adult wing and halter structures, and is regulated by Dpp and Wg signaling. Using a Drosophila invasion model of wing epithelium, we showed herein that inhibition of Dpp or Wg signaling promoted cells to migrate across the cell lineage restrictive anterior/posterior (A/P) compartment boundary. Being downstream of both Dpp and Wg signaling, vg can block cell migration induced by loss of either pathway. In addition, suppression of vg is sufficient to induce cell migration across the A/P boundary. Transcriptomic analysis revealed potential downstream genes involved in the cell migration after suppressing vg in the wing disc. We further demonstrated that the c-Jun N-terminal kinase (JNK) signaling promoted cell migration induced by vg suppression by upregulating Caspase activity. Taken together, our results revealed the requirement of Vg for suppressing cell migration and clarified how developmental signals collaborate to stabilize cells along the compartment boundary.