ASSOCIATION OF THE HERPES-SIMPLEX VIRUS REGULATORY PROTEIN ICP4 WITH SPECIFIC NUCLEOTIDE-SEQUENCES IN DNA
ASSOCIATION OF THE HERPES-SIMPLEX VIRUS REGULATORY PROTEIN ICP4 WITH SPECIFIC NUCLEOTIDE-SEQUENCES IN DNA
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DOI：
10.1093/nar/14.15.6067
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发表时间：
1986-08-11
影响因子：
14.9
通讯作者：
WILCOX, KW
中科院分区：
文献类型：
--
作者：
FABER, SW;WILCOX, KW
我们报告，单纯疱疹病毒（HSV）的转录调节蛋白指定的ICP 4是一个稳定的复合物之间的蛋白质和特定的核苷酸序列的双链DNA形成的外源DNA的一个组成部分，无论是从HSV-1感染的细胞或部分纯化的制备天然ICP 4的粗提物。直接或间接与ICP 4结合的DNA位点被称为ICP 4/蛋白质结合位点。通过DNA酶足迹法鉴定了三个独立的ICP 4/蛋白结合位点;两个在载体pBR 322中，一个位于HSV糖蛋白D mRNA帽位点上游约100个核苷酸处。这三个位点的核苷酸序列的比较揭示了几个同源性区域。我们认为序列5′ATCGTCNNNNYCGRC-3′（N =任意碱基; Y =嘧啶; R =嘌呤）是ICP 4/蛋白结合位点的重要组成部分。
We report that the herpes simplex virus (HSV) transcription regulatory protein designated ICP4 is a component of a stable complex between protein and specific nucleotide sequences in double-stranded DNA formed by addition of exogeneous DNA to either a crude extract obtained from HSV-1 infected cells or a partially purified preparation of native ICP4. DNA sites which are bound directly or indirectly to ICP4 have been designated ICP4/protein binding sites. Three independent ICP4/protein binding sites have been identified by DNAse footprinting; two are in the vector pBR322 and one is located approximately 100 nucleotides upstream from the HSV glycoprotein D mRNA cap site. Comparison of the nucleotide sequences in these three sites reveals several regions of homology. We propose that the sequence 5′ATCGTCNNNNYCGRC-3′ (N = any base; Y = pyrimidine; R = purine) forms an essential component of the ICP4/protein binding site.