ATP‐dependent translocation of amino phospholipids across the human erythrocyte membrane
ATP‐dependent translocation of amino phospholipids across the human erythrocyte membrane
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氨基磷脂跨人红细胞膜的 ATP 依赖性易位
DOI：
10.1016/0014-5793(86)80044-8
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发表时间：
1986
期刊：
影响因子：
3.5
通讯作者：
L. van Deenen
中科院分区：
文献类型：
--
作者：
L. Tilley;S. Cribier;B. Roelofsen;J. den Kamp;L. van Deenen
使用一种非特异性的脂转移蛋白，将微量的放射性标记磷脂插入完整的人红细胞的外膜小叶中。磷脂酰胆碱、磷脂酰丝氨酸和磷脂酰乙醇胺从供体脂泡转移到完整红细胞膜的过程同样容易，而鞘磷脂的转移效率要低6倍。用磷脂酶处理完整细胞，评估标记磷脂的跨双层迁移率和平衡分布。在新鲜红细胞中，标记的氨基磷脂似乎快速地向内小叶移动。另一方面，胆碱磷脂接近于平衡分布，这强烈有利于外层小叶。在耗尽三磷酸腺苷的红细胞中，氨基磷脂的移位明显延迟。
Trace amounts of radiolabeled phospholipids were inserted into the outer membrane leaflet of intact human erythrocytes, using a non‐specific lipid transfer protein. Phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine were transferred from the donor lipid vesicles to the membrane of the intact red cell with equal ease, whilst sphingomyelin was transferred 6‐times less efficiently. The transbilayer mobility and equilibrium distribution of the labeled phospholipids were assessed by treatment of the intact cells with phospholipases. In fresh erythrocytes, the labeled amino phospholipids appeared to move rapidly towards the inner leaflet. The choline phospholipids, on the other hand, approached an equilibrium distribution which strongly favoured the outer leaflet. In ATP‐depleted erythrocytes, the relocation of the amino phospholipids was markedly retarded.
DOI：
--
发表时间：
1982-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
N. Mohandas;J. Wyatt;S. Mel;M. Rossi;S. Shohet
通讯作者：
N. Mohandas;J. Wyatt;S. Mel;M. Rossi;S. Shohet