A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells
A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells
复制标题
石墨烯功能化电化学适体传感器用于选择性无标记检测癌细胞
DOI：
10.1016/j.biomaterials.2011.01.002
复制
发表时间：
2011-04-01
期刊：
影响因子：
14
通讯作者：
Qu, Xiaogang
中科院分区：
文献类型：
--
作者：
Feng, Lingyan;Chen, Yong;Qu, Xiaogang
在这里，我们报道了一种电化学传感器，它可以利用第一个临床试验II使用适体AS1411和功能化石墨烯来实现无标记癌细胞检测。利用AS1411对癌细胞表面过度表达的核仁素的高结合亲和力和特异性，我们开发的电化学适体传感器可以区分癌细胞和正常细胞，并检测低至1000个细胞。通过 DNA 杂交技术，这种 E-DNA 传感器可以再生并可重复用于癌细胞检测。我们的工作为基于适配体和石墨烯修饰电极的无标记癌细胞检测提供了一个很好的例子。 (C) 2011 Elsevier Ltd. 保留所有权利。
Here we report an electrochemical sensor that can realize label-free cancer cell detection using the first clinical trial II used aptamer AS1411 and functionalized graphene. By taking advantages of AS1411 high binding affinity and specificity to the overexpressed nucleolin on the cancer cell surface, our developed electrochemical aptasensor can distinguish cancer cells and normal ones and detect as low as one thousand cells. With DNA hybridization technique, this E-DNA sensor can be regenerated and reusable for cancer cell detection. Our work gives a good example for label-free cancer cell detection based on aptamer and graphene-modified electrode. (C) 2011 Elsevier Ltd. All rights reserved.