RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase
RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase
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DOI：
10.1128/aac.32.11.1684
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发表时间：
1988-11
影响因子：
4.9
通讯作者：
P. Rådström;G. Swedberg
中科院分区：
文献类型：
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作者：
P. Rådström;G. Swedberg
测定了ⅱ型磺胺抗性二氢蝶呤合成酶基因的核苷酸序列。maxicells测定的分子量为30000，多肽的预测分子量为28469。与Tn21编码的sulI基因的DNA相似性为57%。sulI编码的多肽在271个氨基酸中有138个与sulI编码的多肽相同。sulII基因位于多种IncQ（宽宿主范围）质粒和其他小的非共轭抗性质粒上。构建了详细的限制性内切图来比较sulII所在的不同质粒。大结合质粒pGS05和IncQ质粒RSF1010含有相同的sulII基因核苷酸序列。这种类型的磺胺耐药性在革兰氏阴性菌中非常常见，因为它能有效地传播到各种质粒。
The nucleotide sequence of the type II sulfonamide resistance dihydropteroate synthase (sulII) gene was determined. The molecular weight determined by maxicells was 30,000, and the predicted molecular weight for the polypeptide was 28,469. Comparison with the sulI gene encoded by Tn21 showed 57% DNA similarity. The sulII-encoded polypeptide has 138 of 271 amino acids in common with the polypeptide encoded by sulI. The sulII gene is located on various IncQ (broad-host-range) plasmids and other small nonconjugative resistance plasmids. Detailed restriction maps were constructed to compare the different plasmids in which sulII is found. The large conjugative plasmid pGS05 and the IncQ plasmid RSF1010 contained identical nucleotide sequences for the sulII gene. This type of sulfonamide resistance is very frequently found among gram-negative bacteria because of its efficient spread to various plasmids.