Biosynthetic and synthetic AChR sequences to study T cells in myasthenia gravis.
Biosynthetic and synthetic AChR sequences to study T cells in myasthenia gravis.
复制标题
用于研究重症肌无力 T 细胞的生物合成和合成 AChR 序列。
DOI：
10.1111/j.1749-6632.1998.tb10938.x
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发表时间：
1998
影响因子：
5.2
通讯作者：
Conti-Fine,BM
中科院分区：
文献类型：
--
作者：
Diethelm-Okita,B;Wells,G;Kuryatov,A;Okita,D;Howard,J;Lindstrom,J;Conti-Fine,BM
抗原（Ag）特异性T细胞系和克隆是理解在正常和自身免疫应答中T细胞区室中发生的分子相互作用的重要工具。特定于给定Ag的多克隆细胞系的体外繁殖产生富含Ag特异性细胞的T细胞群体，并允许对形成T表位的Ag序列区域进行研究。如果使用自体血CD4+细胞，这种研究的成功率将是可疑的，因为对一个单独的表位具有特异性的T细胞的频率很低。此外，Ag特异性T细胞系和克隆对于研究识别给定Ag或表位的CD4+细胞的功能特性以及其T细胞受体的结构特性是必要的。对于涉及已知身份和序列的自身抗原（autoAg）的人类自身免疫疾病，鉴于相关autoAg的稀缺性，合成和生物合成的autoAg序列已用于繁殖特异性T细胞系。使用合成或重组AChR序列研究了重症肌无力（MG）患者的抗AChR CD4+ T细胞，有时产生不一致的结果，并提出了关于这些AChR Ag用于T细胞研究的适用性的问题。第1、3条
Antigen (Ag)–specific T cell lines and clones are important tools to understand the molecular interactions occurring in the T cell compartment in both normal and autoimmune responses. Propagation in vitro of polyclonal cell lines specific for a given Ag yields populations of T cells enriched in Ag-specific cells and allows studies on the Ag sequence regions forming T epitopes. Such studies would be of dubious success if using unselected blood CD4+ cells, given the low frequency of T cells specific for one individual epitope. 1, 2 Also, Ag-specific T cell lines and clones are necessary to investigate the functional properties of the CD4+ cells recognizing a given Ag or epitope, as well as the structural properties of their T cell receptor. For human autoimmune diseases involving autoantigens (autoAg) of known identity and sequence, given the scarcity of the relevant autoAg, synthetic and biosynthetic autoAg sequences have been used to propagate specific T cell lines. Anti-AChR CD4+ T cells from myasthenia gravis (MG) patients have been studied using synthetic or recombinant AChR sequences, sometimes yielding inconsistent results and raising questions about the suitability of those AChR Ag for the study of T cells. 1, 3