Mixtures of tense and relaxed state polymerized human hemoglobin regulate oxygen affinity and tissue construct oxygenation.
Mixtures of tense and relaxed state polymerized human hemoglobin regulate oxygen affinity and tissue construct oxygenation.
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DOI：
10.1371/journal.pone.0185988
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Palmer AF
中科院分区：
文献类型：
--
作者：
Belcher DA;Banerjee U;Baehr CM;Richardson KE;Cabrales P;Berthiaume F;Palmer AF
合成了纯紧张型(T)和弛豫型(R)四元态聚合人血红蛋白（PolyhHbs），并对其生物物理性质进行了表征，以及T态和R态聚合人血红蛋白的混合物。结果表明，polyhbb混合物的氧亲和力随t态摩尔分数呈线性变化。计算分析了在肝中空纤维（HF）生物反应器中促进单纤维氧化的polyhbb，以评估T态和r态polyhbb混合物的氧化电位。t态摩尔分数大于50%的polyhbb混合物导致缺氧和高氧区占据不到总额外毛细管空间（ECS）的5%。在这些条件下，随着t态摩尔分数从50%增加到100%，ECS中心周围体积与静脉周围体积之比增加了一倍。这些结果显示了不同T/ r态polyhbb摩尔分数对组织工程构建物氧合的影响及其对组织氧合的潜力。
Pure tense (T) and relaxed (R) quaternary state polymerized human hemoglobins (PolyhHbs) were synthesized and their biophysical properties characterized, along with mixtures of T- and R-state PolyhHbs. It was observed that the oxygen affinity of PolyhHb mixtures varied linearly with T-state mole fraction. Computational analysis of PolyhHb facilitated oxygenation of a single fiber in a hepatic hollow fiber (HF) bioreactor was performed to evaluate the oxygenation potential of T- and R-state PolyhHb mixtures. PolyhHb mixtures with T-state mole fractions greater than 50% resulted in hypoxic and hyperoxic zones occupying less than 5% of the total extra capillary space (ECS). Under these conditions, the ratio of the pericentral volume to the perivenous volume in the ECS doubled as the T-state mole fraction increased from 50 to 100%. These results show the effect of varying the T/R-state PolyhHb mole fraction on oxygenation of tissue-engineered constructs and their potential to oxygenate tissues.
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