Active reassortment of H9 influenza viruses between wild birds and live-poultry markets in Korea
Active reassortment of H9 influenza viruses between wild birds and live-poultry markets in Korea
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DOI：
10.1007/s00705-009-0577-4
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发表时间：
2010-02-01
影响因子：
2.7
通讯作者：
Kim, Chul Joong
中科院分区：
文献类型：
--
作者：
Moon, Ho Jin;Song, Min Suk;Kim, Chul Joong
从2004年9月至2007年10月对韩国活禽市场的H9禽流感病毒进行了监测，以调查韩国野生候鸟与家禽之间的活跃重组。抗原性和系统发育分析表明，大多数分离株属于先前的韩国h9n2样谱系，与中国东南部菌株不同。有趣的是，Ck/Korea/LPM77/06组（基因型B）和Dk/Korea/LPM248/07组（基因型C）表现出不同于其他韩国H9N2株的独特特性。虽然这两组的HA基因属于韩国的h9n2样谱系，但PA基因与中国的y280样谱系非常相似。另外，Dk/Korea/LPM248/07群体的PB2基因与候鸟分离的PB2基因有密切的亲缘关系。其他几个分离株也聚集在H9N2 B基因型内，这表明韩国至少有两种主要的H9N2流感基因型。另一株分离株Dk/Korea/LPM71/06被确定为h5n1亚型，这是在韩国活禽市场首次发现的。这些发现表明，韩国H9流感病毒重组在活禽市场经常发生，可能是由候鸟之间传播的病毒遗传物质传入家禽介导的。因此，通过持续的重组，新的H9N2显性基因型已经在韩国活禽市场建立起来。
Surveillance of H9 avian influenza viruses in Korean live-poultry markets from September 2004 through October 2007 was carried out to investigate active reassortment between wild migratory birds and domestic poultry in Korea. Antigenic and phylogenetic analyses showed that most of the isolates belong to the previous Korean H9N2-like lineage and differ from the southeastern Chinese strains. Interestingly, the Ck/Korea/LPM77/06 group (genotype B) and Dk/Korea/LPM248/07 group (genotype C) showed unique properties distinct from those of other Korean H9N2 strains. Although the HA genes of these two groups belong to Korean H9N2-like lineage, the PA genes closely resemble those of the Chinese Y280-like lineage. In addition, the PB2 genes of the Dk/Korea/LPM248/07 group were closely related to those isolated from migratory birds. Several other isolates also clustered within the H9N2 B genotype, an indication that there are at least two predominant H9N2 influenza genotypes in Korea. Another isolate, Dk/Korea/LPM71/06, was identified as an H9N1 subtype, the first ever discovered in Korean live-poultry markets. These findings reveal that reassortment of Korean H9 influenza viruses has occurred frequently in live-poultry markets and may have been mediated by introduction of genetic material from viruses circulating among migratory wild birds to domestic birds. Consequently, the new dominant H9N2 genotypes have become established in Korean live-poultry markets through continued reassortment.