Assessment of functional relevance of genes associated with local temperature variables in Arabidopsis thaliana
Assessment of functional relevance of genes associated with local temperature variables in Arabidopsis thaliana
复制标题
拟南芥局部温度变量相关基因的功能相关性评估
DOI：
10.1111/pce.14417
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hua, Jian
中科院分区：
文献类型：
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作者：
Jiang, Yuan;Wang, Zhixue;Du, Hui;Dong, Runlong;Yuan, Yaping;Hua, Jian
通过全基因组关联研究在计算机中识别出的与环境相关的遗传变异在功能上与环境适应相关的可能性在很大程度上尚未通过实验进行探索。在这里，我们分析了 1129 个拟南芥天然种质中含有与局部温度变化（最小值、平均值、最大值）相关的多态性的前 29 个基因。他们的功能丧失突变体在五个温度下的生长和应激耐受性进行了评估。发现二十个基因会影响这些温度中的一种或多种的生长或耐受性。值得注意的是，与最高温度相关的基因更有可能在较高温度下具有检测功能，而与最低温度相关的基因更有可能在较低温度下具有检测功能。此外，基因变异更频繁地分布在这些地理位置，这些地理位置显然可以增强对所测试的五个基因的生长或耐受性。此外，大部分计算机识别的与最低或平均温度相关的基因的变化表现出与一小部分自然种质在低温下实验评估的生长表型的显着相关性。这项研究显示了与环境变量相关的基因变异的功能相关性，并支持使用当地温度因素来研究温度适应的遗传基础的可行性。
How likely genetic variations associated with environment identified in silico from genome wide association study are functionally relevant to environmental adaptation has been largely unexplored experimentally. Here we analyzed top 29 genes containing polymorphisms associated with local temperature variation (minimum, mean, maximum) among 1129 natural accessions ofArabidopsis thaliana. Their loss‐of‐function mutants were assessed for growth and stress tolerance at five temperatures. Twenty genes were found to affect growth or tolerance at one or more of these temperatures. Significantly, genes associated with maximum temperature more likely have a detect a function at higher temperature, while genes associated with minimum temperature more likely have a function at lower temperature. In addition, gene variants are distributed more frequently at geographic locations where they apparently offer an enhanced growth or tolerance for five genes tested. Furthermore, variations in a large proportion of the in silico identified genes associated with minimum or mean‐temperatures exhibited a significant association with growth phenotypes experimentally assessed at low temperature for a small set of natural accessions. This study shows a functional relevance of gene variants associated with environmental variables and supports the feasibility of the use of local temperature factors in investigating the genetic basis of temperature adaptation.