Morphogenetic activity of extracellular matrices on cultured podocytes. Laminin accelerates podocyte process formation in vitro.
Morphogenetic activity of extracellular matrices on cultured podocytes. Laminin accelerates podocyte process formation in vitro.
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细胞外基质对培养足细胞的形态发生活性。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
S. Matsuda
中科院分区：
文献类型：
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作者：
N. Kobayashi;K. Mominoki;H. Wakisaka;Y. Shimazaki;S. Matsuda
使用条件永生化足细胞系研究了各种细胞外基质蛋白对肾脏足细胞的形态发生作用。将足细胞铺在玻璃盖玻片上并用以下基质蛋白包被：层粘连蛋白-10/11、层粘连蛋白-1、纤连蛋白、IV型胶原、I型胶原。接种后3小时，层粘连蛋白上的足细胞形成突出的突起，而其他基质蛋白上的足细胞在2天后开始伸长突起。粘蛋白免疫标记显示，足细胞板层粘连蛋白具有薄棒形的焦点接触，而那些纤维连接蛋白显示大点状的焦点接触。丝氨酸/苏氨酸蛋白激酶的抑制诱导足细胞的过程中形成的细胞外基质的独立方式。本研究揭示了层粘连蛋白在体外足细胞形态发生中的意义，并表明不同的细胞外基质蛋白触发不同的细胞内信号支配足细胞形态发生。结合我们以前的研究，足细胞突起的形成被认为是由蛋白质Ser/Thr磷酸化调节的。
Morphogenetic effects of various extracellular matrix proteins on the renal podocyte were investigated using the conditionally immortalized podocyte cell line. Podocytes were plated on glass coverslips and coated with the following matrix proteins: laminin-10/11, laminin-1, fibronectin, collagen type IV, collagen type I. Three hours after plating, podocytes on laminins developed prominent processes, while those on other matrix proteins started to elongate processes after two days. Vinculin-immunolabeling showed that podocytes plated on laminins possessed thin rod-shaped focal contacts, whereas those on fibronectin showed large dot-shaped focal contacts. Inhibition of serine/threonine protein kinases induced podocyte process formation in an extracellular matrix-independent manner. The present study reveals the significance of laminin on podocyte morphogenesis in vitro, and shows that different extracellular matrix proteins trigger different intracellular signals governing podocyte morphogenesis. Taken together with our previous studies, podocyte process formation is thought to be regulated by protein Ser/Thr phosphorylation.