The Mode of Biofilm Formation on Smooth Surfaces by Campylobacter jejuni
The Mode of Biofilm Formation on Smooth Surfaces by Campylobacter jejuni
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DOI：
10.1292/jvms.09-0339
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发表时间：
2010-04-01
影响因子：
1.2
通讯作者：
Misawa, Naoaki
中科院分区：
文献类型：
--
作者：
Moe, Kyaw Kyaw;Mimura, Junichiro;Misawa, Naoaki
许多微生物产生细胞外聚合物，统称为“粘液”或糖萼。并在自然生态系统的固体表面形成生物膜。空肠弯曲菌是最重要的食源性致病菌之一。也具有在体外在不锈钢、玻璃或聚氯乙烯上形成生物膜的能力。然而，弯曲杆菌属物种生物膜形成的问题尚未得到广泛研究。本研究旨在探讨C.空肠至光滑表面。当将布鲁氏菌肉汤中的细菌悬液在带有盖玻片的微孔板威尔斯孔中孵育时，在孵育开始后2小时内在盖玻片上形成直径为0.5-2 mm的小菌落。这些小菌落逐渐生长，并形成了一个生物膜的网状连接6小时内。透射电子显微镜表明，大量的细胞外物质掩盖了细胞表面。并且该材料结合钌红，表明存在多糖部分。扫描电子显微镜观察表明，鞭毛起桥梁作用，在生物体之间形成网状连接。为了确定与生物膜形成相关的基因，通过自然转化介导的等位基因交换从菌株81-176构建了无鞭毛（flaA）和有鞭毛但不运动（motA）突变体。flaA（-）和motA（-）突变体不形成野生型菌株所表现出的生物膜。这些发现表明，鞭毛介导的运动，以及鞭毛是所需的生物膜形成在体外。
Many microorganisms produce extracellular polymers referred to collectively as "slime" or glycocalyx. and form biofilms on solid surfaces in natural ecosystems. Campylobacter jejuni, one of the most important foodborne pathogens. also has the ability to form biofilm on stainless steel, glass, or polyvinyl chloride in vitro. However, the issue of biofilm formation by Campylobacter species has not been extensively examined. The present study was performed to examine the mode of adhesion of C. jejuni to a smooth surface. When bacterial suspensions in Brucella broth were incubated in microplate wells with a glass coverslip, microcolonies 0.5-2 mm in diameter were formed on the coverslip within 2 hr from the start of incubation. These microcolonies gradually grew and formed a biofilm of net-like connections within 6 hr. Transmission electron microscopy indicated that massive amounts of extracellular material masked the cell surface. and this material bound ruthenium red, suggesting the presence of a polysaccharide moiety. Scanning electron microscopy indicated that the flagella acted as bridges, forming net-like connections between the organisms. To determine the genes associated with biofilm formation, aflagellate (flaA) and flagellate but non-motile (motA) mutants were constructed from strain 81-176 by natural transformation-mediated allelic exchange. The flaA(-) and motA(-) mutants did not form the biofilm exhibited by the wild-type strain. These findings suggest that flagella-mediated motility as well as flagella is required for biofilm formation in vitro.