Direct in vivo measurement of human cerebral GABA concentration using MEGA-editing at 7 Tesla
Direct in vivo measurement of human cerebral GABA concentration using MEGA-editing at 7 Tesla
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DOI：
10.1002/mrm.10146
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发表时间：
2002-05-01
影响因子：
3.3
通讯作者：
Gruetter, R
中科院分区：
文献类型：
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作者：
Terpstra, M;Ugurbil, K;Gruetter, R
GABA自旋系统的光谱编辑受到大分子（MM）内聚物的共同编辑的阻碍。为了减少由GABA编辑的Bisocin光谱引起的污染，使用了目前可用于人体实验的最高场强（7特斯拉）和基于MEGA的编辑。尽管明智的选择实验参数，MM污染被发现产生的领域漂移。当考虑MM贡献时，相对于8 mumol/g肌酸（Cr），[GABA] = 0.75 +/- 0.14 mumol/g（平均值+/- SD，N = 16），而不考虑MM信号[GABA*] = 0.88 +/- 0.23 mumol/g（平均值SD，N = 16）。将MM污染的直接实验评估简化为编辑的GABA信号大大降低了测量的方差，导致浓度与先前的C-13标记实验非常一致。(C)2002 Wiley-Liss，Inc.
Spectral editing of the GABA spin system is hampered by co-editing of macromolecule (MM) coherences. To reduce contamination arising from MMs in spectra edited for GABA, the highest field strength currently available for human experimentation (7 Tesla) and MEGA-based editing were used. Despite judicious choice of experimental parameters, MM contamination was found to arise from field drifts. When the MM contribution was accounted for, [GABA] = 0.75 +/- 0.14 mumol/g (mean +/- SD, N = 16) relative to 8 mumol/g creatine (Cr), whereas without accounting for the MM signal [GABA*] = 0.88 +/- 0.23 mumol/g (mean SD, N = 16). Incorporating the direct experimental assessment of MM contamination to the edited GABA signal substantially reduced the variance of the measurement, resulting in concentrations that were in excellent agreement with previous C-13 labeling experiments. (C) 2002 Wiley-Liss, Inc.