Alternative 3'-end processing of U5 snRNA by RNase III
Alternative 3'-end processing of U5 snRNA by RNase III
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DOI：
10.1101/gad.11.20.2741
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发表时间：
1997-10-15
影响因子：
10.5
通讯作者：
Guthrie, C
中科院分区：
文献类型：
--
作者：
Chanfreau, G;AbouElela, S;Guthrie, C
形成小核rna的3'端所需的细胞成分是未知的。酿酒酵母的U5 snRNA在其3'端有两种长度不同的形式（U5L和U5S）。当添加到酵母细胞无提取物时，合成的含有下游基因组序列的pre-U5 RNA被有效而准确地处理，以产生两种成熟形式的U5。这两种形式的U5是通过3'端交替加工在体外产生的。编码RNase III的RNT1基因的温度敏感突变可阻断体内U5L的积累。在体外，RNase III对U5前体的选择性切割决定了U5L和U5S的两个多步骤途径之间的选择，其中一个（U5L）严格依赖于RNase III。这些结果确定了RNase III是参与snRNA 3'端形成的反式作用因子，并显示了RNase III如何调节其他RNA加工途径。
The cellular components required to form the 3' ends of small nuclear RNAs are unknown. U5 snRNA from Saccharomyces cerevisiae is found in two forms that differ in length at their 3' ends (U5L and U5S). When added to a yeast cell free extract, synthetic pre-U5 RNA bearing downstream genomic sequences is processed efficiently and accurately to generate both mature forms of U5. The two forms of U5 are produced in vitro by alternative 3'-end processing. A temperature-sensitive mutation in the RNT1 gene encoding RNase III blocks accumulation of U5L in vivo. In vitro, alternative cleavage of the U5 precursor by RNase III determines the choice between the two multistep pathways that lead to U5L and U5S, one of which (U5L) is strictly dependent on RNase III. These results identify RNase III as a trans-acting factor involved in 3'-end formation of snRNA and show how RNase III might regulate alternative RNA processing pathways.