Learning of probabilistic punishment as a model of anxiety produces changes in action but not punisher encoding in the dmPFC and VTA.
Learning of probabilistic punishment as a model of anxiety produces changes in action but not punisher encoding in the dmPFC and VTA.
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DOI：
10.7554/elife.78912
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发表时间：
2022-09-14
期刊：
影响因子：
7.7
通讯作者：
Moghaddam B
中科院分区：
文献类型：
--
作者：
Jacobs DS;Allen MC;Park J;Moghaddam B
此前，我们通过训练大鼠执行一项任务开发了一种在动机行为期间的焦虑新模型，在该任务中，为获取奖励而执行的动作有一定概率会受到惩罚，并且观察到在学习之后，腹侧被盖区（VTA）和背内侧前额叶皮层（dmPFC）的神经元活动代表了动作与惩罚风险之间的关系（帕克和莫哈达姆，2017年）。在此，我们使用雄性和雌性大鼠对先前的工作进行拓展，重点关注与概率性惩罚学习相关的dmPFC和VTA中的神经变化，以及学习后用地西泮进行的抗焦虑治疗。我们发现，在学习致焦虑的偶然事件过程中，dmPFC和VTA的适应性神经反应与惩罚者的经历无关，且主要发生在动作前后和奖励阶段。我们的研究结果还确定了VTA神经钙活动在动作前后的上升，以及VTA - dmPFC的相关活动，作为地西泮抗焦虑特性的潜在标志物。
Previously, we developed a novel model for anxiety during motivated behavior by training rats to perform a task where actions executed to obtain a reward were probabilistically punished and observed that after learning, neuronal activity in the ventral tegmental area (VTA) and dorsomedial prefrontal cortex (dmPFC) represent the relationship between action and punishment risk (Park and Moghaddam, 2017). Here, we used male and female rats to expand on the previous work by focusing on neural changes in the dmPFC and VTA that were associated with the learning of probabilistic punishment, and anxiolytic treatment with diazepam after learning. We find that adaptive neural responses of dmPFC and VTA during the learning of anxiogenic contingencies are independent from the punisher experience and occur primarily during the peri-action and reward period. Our results also identify peri-action ramping of VTA neural calcium activity, and VTA-dmPFC correlated activity, as potential markers for the anxiolytic properties of diazepam.