CODON AND AMINO-ACID SPECIFICITIES OF A TRANSFER-RNA ARE BOTH CONVERTED BY A SINGLE POST-TRANSCRIPTIONAL MODIFICATION
CODON AND AMINO-ACID SPECIFICITIES OF A TRANSFER-RNA ARE BOTH CONVERTED BY A SINGLE POST-TRANSCRIPTIONAL MODIFICATION
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DOI：
10.1038/336179a0
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发表时间：
1988-11-10
期刊：
影响因子：
64.8
通讯作者：
YOKOYAMA, S
中科院分区：
文献类型：
--
作者：
MURAMATSU, T;NISHIKAWA, K;YOKOYAMA, S
AnEscherichia coliisoleucine transfer RNA specific for the codon AUA (tRNA2Ileor tRNAminorIle(ref. 1) has a novel modified nucleo-side, lysidine (L; ref. 2) (Fig. la) in the first position of the anticodon (position 34), which is essential for the specific recognition of the codon AUA (ref. 1). We isolated the gene for tRNA2Ile(ileX)and found that the anticodon is CAT, which is characteristic of the methionine tRNA gene. Replacement of L(34) of tRNA2Ilemolecule enzymatically with unmodified C(34) resulted in a marked reduction of the isoleucine-accepting activity and, surprisingly, in the appearance of methionine-accepting activity. Thus, both the codon and amino-acid specificity of this tRNA are converted by a single post-transcriptional modification of the first position of the anticodon during tRNA maturation.
