Ionic liquids as additives in the pig liver esterase (PLE) catalysed synthesis of chiral disubstituted malonates
Ionic liquids as additives in the pig liver esterase (PLE) catalysed synthesis of chiral disubstituted malonates
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离子液体作为猪肝酯酶（PLE）催化合成手性二取代丙二酸酯的添加剂
DOI：
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
C. Bolm
中科院分区：
文献类型：
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作者：
Stefan Wallert;K. Drauz;I. Grayson;H. Gröger;P. D. María;C. Bolm
本文首次将羟基化和环氧化离子液体作为添加剂，以猪肝酯酶（PLE）为催化剂，对丙二酸二酯进行了对映选择性水解。不到1%的亲水性功能化离子液体，与10%的异丙醇/水混合使用，就足以将酶的活性提高4倍，并增加反应的对映选择性。将PLE固定在Eupergit®C上，可以成功地回收酶系统。
This report describes the first application of hydroxylated and epoxylated ionic liquids as additives in the enantioselective hydrolysis of prochiral diester malonates using pig liver esterase (PLE) as catalyst. Less than 1% of a hydrophilic functionalised ionic liquid, used in combination with 10% isopropanol/water is sufficient to enhance the activity of the enzyme by a factor of up to 4 and also to increase the enantioselectivity of the reaction. Immobilisation of the PLE on Eupergit® C enabled successful recycling of the enzyme system.