Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development
Fish and Chips: a microfluidic perfusion platform for monitoring zebrafish development
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DOI：
10.1039/c1lc20351g
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发表时间：
2012-01-01
期刊：
影响因子：
6.1
通讯作者：
Yu, Hanry
中科院分区：
文献类型：
--
作者：
Choudhury, Deepak;van Noort, Danny;Yu, Hanry
我们开发了一种多通道微流控灌注平台，用于培养斑马鱼胚胎并捕获胚胎内各种组织和器官的实时图像。鱼和芯片是在硅和玻璃微制造的微流体结构的再现性和准确性。微流控平台由三部分组成：微流控梯度发生器、一排八个鱼缸（鱼胚胎单独放置在其中）和八个输出通道。流体梯度发生器支持剂量依赖性药物和化学研究。独特的灌注系统确保了介质均匀和恒定地流入鱼缸，同时有效地去除废物。鱼缸限制胚胎的运动，除了旋转，用于内部组织和器官的实时成像。胚胎在药物丙戊酸（VPA）的影响下表现出发育异常。
We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA).