Experimental infection of inbred mice with herpes simplex virus
Experimental infection of inbred mice with herpes simplex virus
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单纯疱疹病毒近交系小鼠的实验感染
作者：
H. Engler;R. Zawatzky;H. Kirchner;D. Armerding
近交系小鼠对单纯疱疹病毒1型或2型(HSV-1、HSV-2)感染的易感性不同。本研究检测了小鼠腹腔注射干扰素后腹腔液中干扰素的产生。注射HSV-1或HSV-2。在HSV耐药小鼠(C57BL/6、C3H/HeJ)中，注射后2~4小时已出现高滴度的干扰素。相比之下，抵抗力较低的小鼠(DBA/2，AKR)缺乏这种早期反应。在感染后12小时后，干扰素效价与耐药性之间没有相关性。12小时后，DBA/2组小鼠腹膜中检测到高滴度的HSV，而C57BL/6组小鼠腹膜中HSV滴度显著降低。在对8个不同近交系小鼠品系的比较分析中，早期(2至4小时)干扰素的产生与耐药性有关。在HSV刺激的早期(24小时)NK细胞反应的检测中，不仅干扰素产生良好的菌株，而且抗药性较差的低干扰素产生株(BALB/c)都显示出显着的细胞毒活性。相反，HSV诱导的NK细胞活性非常低的SJL小鼠具有抵抗力，并在局部部位表现出高早期干扰素反应。
SummaryInbred mouse strains differ in susceptibility to infection with herpes simplex virus type 1 or type 2 (HSV-1, HSV-2). In this study interferon production was tested in the peritoneal exudate of mice after intraperitoneal (i.p.) injection of HSV-1 or HSV-2. In HSV-resistant mice (C57BL/6, C3H/HeJ) high titers of interferon were already present 2 to 4 hours after injection. In comparison, less resistant mice (DBA/2, AKR) lacked this early response. There was no correlation between interferon titers and resistance at post-infection times later than twelve hours. At twelve hours, however, high titers of HSV were detected in the peritoneum of DBA/2 mice and significantly lower titers in C57BL/6 mice. In a comparative analysis of eight different inbred mouse strains, again early (2 to 4 hours) interferon production was correlated to resistance. In assays of HSV-stimulated early (24 hours) NK cell responses not only the good interferon producer strains but also one of the less resistant low interferon producers (BALB/c) showed significant cytotoxic activities. Conversely, SJL mice that are very low in HSV-induced NK cell activity are resistant and show high early interferon responses at the local site.