Non-covalently functionalized single-walled carbon nanotube for topical siRNA delivery into melanoma
Non-covalently functionalized single-walled carbon nanotube for topical siRNA delivery into melanoma
复制标题
用于局部 siRNA 递送至黑色素瘤的非共价功能化单壁碳纳米管
DOI：
10.1016/j.biomaterials.2013.12.079
复制
发表时间：
2014-03-01
期刊：
影响因子：
14
通讯作者：
Min, Wei-Ping
中科院分区：
文献类型：
--
作者：
Siu, King Sun;Chen, Di;Min, Wei-Ping
RNAi可以特异性调节基因表达，但siRNA在体内的有效递送是困难的，而修饰的碳纳米管（CNT）已被证明可以保护siRNA，促进进入细胞并增强经皮药物递送。单壁碳纳米管（SWCNT）与琥珀化聚乙烯亚胺（PEI-SA）进行了非共价官能化。本研究分离并表征了水溶性CNT、PEI-SA/CNT (IS/C)，并在体外实现了IS/C/siRNA复合物对B16-F10细胞的基因沉默作用。体内递送局部应用于剃光的小鼠皮肤，以及局部应用于C57BL/6小鼠黑色素瘤模型。我们发现肿瘤组织中cy3标记的Braf特异性siRNA （siBraf）的显著摄取和基因沉默。使用IS/C/siBraf治疗可在25天内抑制肿瘤生长。这种新的递送方法为未来siRNA的递送和治疗提供了新的可能性，为基于碳纳米管的siRNA递送的潜在应用和发展提供了见解。(C) 2013 Elsevier Ltd.版权所有。
RNAi can specifically regulate gene expression, but efficient delivery of siRNA in vivo is difficult while it has been shown that modified carbon nanotubes (CNT) protect siRNA, facilitate entry into cells and enhance transdermal drugs delivery. Single-walled carbon nanotubes (SWCNT) were functionalized non-covalently with succinated polyethyleimine (PEI-SA). In this study, the water soluble CNT, PEI-SA/CNT (IS/C) were isolated and characterized, the gene silencing induced by IS/C/siRNA complexes was achieved in vitro in B16-F10 cells. In vivo delivery was topically applied to shaved mouse skin, as well as topically to a C57BL/6 mice melanoma model. We found significant uptake of Cy3-labeled siRNA specific to Braf (siBraf) and gene silencing in the tumor tissue. Treatment with IS/C/siBraf resulted in attenuation of tumor growth over a 25-day period. This new delivery method has provided a new possibility for future siRNA delivery and therapy, which providing insight for the potential application and development of CNT-based siRNA delivery. (C) 2013 Elsevier Ltd. All rights reserved.