Characterization and autoregulation of latent transforming growth factor beta (TGF beta) complexes in osteoblast-like cell lines. Production of a latent complex lacking the latent TGF beta-binding protein.
Characterization and autoregulation of latent transforming growth factor beta (TGF beta) complexes in osteoblast-like cell lines. Production of a latent complex lacking the latent TGF beta-binding protein.
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DOI：
10.1016/s0021-9258(17)37449-5
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发表时间：
1994-03
期刊：
影响因子：
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通讯作者：
S. L. Dallas;S. Park-Snyder;K. Miyazono;D. Twardzik;G. R. Mundy;L. F. Bonewald
中科院分区：
文献类型：
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作者：
S. L. Dallas;S. Park-Snyder;K. Miyazono;D. Twardzik;G. R. Mundy;L. F. Bonewald
我们以前已经表明，骨器官培养产生大量的潜在转化生长因子β（TGF β），它缺乏潜在的TGF β结合蛋白（LTBP）。在这项研究中，我们使用已知的成骨细胞样细胞系UMR-106，ROS 17/2.8和MG 63作为模型，以进一步研究骨中潜在的TGF β表达。我们发现骨肉瘤细胞系UMR-106几乎完全以缺乏LTBP的100-kDa复合物的形式分泌潜伏的TGF β。ROS 17/2.8细胞产生100-kDa复合物和含有成纤维细胞（190 kDa）形式的LTBP的290-kDa复合物。MG 63细胞（如人包皮成纤维细胞）几乎只表达290-kDa复合物。为了研究骨细胞中潜在TGF β复合物的调节，我们评估了TGF β 1处理对活性和潜在TGF β表达的影响。TGF β 1在所有检查的细胞类型中诱导潜伏但非活性TGF β的分泌。在人包皮成纤维细胞中，TGF β 1和LTBP mRNA同时表达。相反，在骨肉瘤细胞系中，TGF β 1 mRNA的自身诱导与LTBP mRNA的延迟增加或无变化相关。在UMR-106细胞中，LTBP信息几乎检测不到。我们假设成骨细胞样细胞表达不同的潜在TGF β形式可能反映了它们的成熟状态，并且不同的潜在TGF β复合物可能具有不同的功能，例如作为分泌形式或作为基质储存形式。
We have previously shown that bone organ cultures produce large amounts of latent transforming growth factor beta (TGF beta), which lacks latent TGF beta-binding protein (LTBP). In this study we used the known osteoblast-like cell lines UMR-106, ROS 17/2.8, and MG63 as models to further examine latent TGF beta expression in bone. We found that the osteosarcoma cell line UMR-106 secreted latent TGF beta almost exclusively as a 100-kDa complex lacking LTBP. ROS 17/2.8 cells produced both the 100-kDa complex and also a 290-kDa complex containing the fibroblastic (190 kDa) form of LTBP. MG63 cells (like human foreskin fibroblasts) expressed almost exclusively the 290-kDa complex. To investigate the regulation of latent TGF beta complexes in bone cells we assessed the effects of TGF beta 1 treatment on expression of active and latent TGF beta. TGF beta 1 induced secretion of latent but not active TGF beta in all cell types examined. In human foreskin fibroblast cells, TGF beta 1 and LTBP mRNA were expressed concomitantly. In contrast, in osteosarcoma cell lines autoinduction of TGF beta 1 mRNA was associated with either a delayed increase or no change in LTBP mRNA. In UMR-106 cells LTBP message was virtually undetectable. We postulate that the expression of different latent TGF beta forms by osteoblast-like cells may reflect their maturation states and that different latent TGF beta complexes may have different functions, for example as secretory forms or as matrix storage forms.