Sickle cell detection using a smartphone.
Sickle cell detection using a smartphone.
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DOI：
10.1038/srep15022
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发表时间：
2015-10-22
影响因子：
4.6
通讯作者：
Tasoglu S
中科院分区：
文献类型：
--
作者：
Knowlton SM;Sencan I;Aytar Y;Khoory J;Heeney MM;Ghiran IC;Tasoglu S
镰状细胞病影响着生活在中非和西非的25%的人，如果不加以诊断，可能导致危及生命的“无声”中风和终身损害。然而，无处不在的检测程序尚未在这些地区实施，需要一个简单，快速和准确的检测平台来诊断镰状细胞病。在这里，我们提出了一个无标记的，灵敏的，特异性的测试平台，仅使用少量的血液样本（<1 μl），基于在缺氧条件下镰状红细胞的较高密度。测试使用安装在商用智能手机上的轻便紧凑的3D打印附件进行。该附件包括一个照明样品的LED、一个放大图像的光学透镜和两个用于红细胞磁悬浮的永磁体。将样品悬浮在含有焦亚硫酸钠的顺磁性介质中，并加载到插入磁体之间的微毛细管中。基于作用在细胞上的磁力和浮力之间的平衡，红细胞在磁场中悬浮。使用这种方法，我们能够区分镰状红细胞与对照红细胞的悬浮模式，基于它们的限制程度。
Sickle cell disease affects 25% of people living in Central and West Africa and, if left undiagnosed, can cause life threatening “silent” strokes and lifelong damage. However, ubiquitous testing procedures have yet to be implemented in these areas, necessitating a simple, rapid, and accurate testing platform to diagnose sickle cell disease. Here, we present a label-free, sensitive, and specific testing platform using only a small blood sample (<1 μl) based on the higher density of sickle red blood cells under deoxygenated conditions. Testing is performed with a lightweight and compact 3D-printed attachment installed on a commercial smartphone. This attachment includes an LED to illuminate the sample, an optical lens to magnify the image, and two permanent magnets for magnetic levitation of red blood cells. The sample is suspended in a paramagnetic medium with sodium metabisulfite and loaded in a microcapillary tube that is inserted between the magnets. Red blood cells are levitated in the magnetic field based on equilibrium between the magnetic and buoyancy forces acting on the cells. Using this approach, we were able to distinguish between the levitation patterns of sickle versus control red blood cells based on their degree of confinement.