Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer.
Expression of I-Ak class II genes in mouse L cells after DNA-mediated gene transfer.
复制标题
DNA 介导的基因转移后小鼠 L 细胞中 I-Ak II 类基因的表达。
作者：
Malissen,B;Steinmetz,M;McMillan,M;Pierres,M;Hood,L
小鼠 H-2 复合体的 I 区编码 I 区相关抗原或 Ia 抗原，这些抗原参与淋巴细胞相互作用和免疫反应的调节。已鉴定出两种 Ia 抗原，称为 I-A 和 I-E；两者都是细胞表面整合膜糖蛋白，由两条非共价连接的多肽链组成，分别命名为α（分子量（MW）∼34,000）和β（∼28,000 MW）1。它们的表达仅限于 B 淋巴细胞和抗原呈递细胞，例如巨噬细胞。在细胞内部，这些αβ二聚体与一条 31,000 MW 的非多态性链非共价结合，称为不变 (Ii) 链2。我们的实验室最近完成了从 H-2d 和 H-2 卡倍型小鼠中克隆 200 KB 的 I 区 3,4，并鉴定了编码四种多肽链 Aα、Aβ、Eα 和 Eβ 的基因。我们现在描述 I-Akm 分子在转染的小鼠 L 细胞中的成功表达。
TheI–region of the mouseH-2complex encodesI-region-associated or Ia antigens which are involved in lymphocyte interactions and in the regulation of immune responsiveness. Two Ia antigens, termed I–A and I–E, have been identified; both are cell-surface integral membrane glycoproteins which consist of two noncovalently linked polypeptide chains designatedα(molecular weight (MW) ∼ 34,000) andβ(∼ 28,000 MW)1. Their expression is restricted to B lymphocytes and to antigen-presenting cells such as macrophages. Inside the cell, theseαβdimers are associated noncovalently with a nonpolymorphic chain of 31,000 MW designated the invariant (Ii) chain2. Cloning of 200 kilobases (kb) of theI-region from mice of theH–2dandH–2khaplotypes was accomplished recently in our laboratory3,4, and the genes encoding the four polypeptide chains, Aα, Aβ, Eαand Eβwere identified. We now describe the successful expression of I–Akmolecules in transfected mouse L cells.