ACTION OF A BACTERIAL AGARASE ON AGAROSE PORPHYRAN AND ALKALI-TREATED PROPHYRAN
ACTION OF A BACTERIAL AGARASE ON AGAROSE PORPHYRAN AND ALKALI-TREATED PROPHYRAN
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DOI：
10.1042/bj1130687
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发表时间：
1969-01-01
影响因子：
4.1
通讯作者：
TURVEY, JR
中科院分区：
文献类型：
--
作者：
DUCKWORTH, M;TURVEY, JR
1.使用来自噬细胞物种的纯化胞外琼脂酶来水解琼脂糖、卟啉和碱处理的卟啉。 2.通过凝胶过滤将琼脂糖的水解产物分离成一系列新琼脂糖，其中主要成员是四糖。 3.酶对碱处理的卟啉的作用产生新琼脂糖和其他含有6-O-甲基-d-半乳糖单元的寡糖。从这些寡糖的组成可以推断，酶对ad-半乳糖苷键的作用比对6-O-甲基-d-半乳糖苷键的作用快四到五倍。 4.酶对天然卟啉的作用产生了一系列相似的寡糖，但产量较小，大部分多糖要么没有降解，要么仅降解为一系列大的、高度硫酸化的寡糖。 5.对于卟啉，得出的结论是6-O-甲基醚基团随机分布在半乳糖单元上，但1-半乳糖单元上的6-硫酸基团倾向于以嵌段形式分布。
1. A purified extracellular agarase from aCytophagaspecies was used to hydrolyse agarose, porphyran and alkali-treated porphyran. 2. The hydrolysate from agarose was separated by gel filtration into the series of neoagarosaccharides, the predominant member of which was the tetrasaccharide. 3. Enzyme action on alkali-treated porphyran gave neoagarosaccharides and other oligosaccharides containing 6-O-methyl-d-galactose units. From the composition of these oligosaccharides it is deduced that action of the enzyme on ad-galactosidic linkage is four to five times faster than on the 6-O-methyl-d-galactosidic linkage. 4. Enzyme action on native porphyran gives a similar series of oligosaccharides but in smaller yield, much of the polysaccharide being either not degraded or only degraded to a series of large, highly sulphated oligosaccharides. 5. For porphyran, it is concluded that 6-O-methyl ether groups are distributed randomly on half thed-galactose units, but that the 6-sulphate groups onl-galactose units tend to occur in blocks.