Combined effects of klotho and soluble CD40 ligand on A549 lung cancer cells
Combined effects of klotho and soluble CD40 ligand on A549 lung cancer cells
复制标题
Klotho和可溶性CD40配体对A549肺癌细胞的联合作用
DOI：
10.3892/or.2011.1178
复制
发表时间：
2011-05-01
期刊：
影响因子：
4.2
通讯作者：
Wu, Jianqing
中科院分区：
文献类型：
--
作者：
Wang, Xueli;Chen, Bo;Wu, Jianqing
尽管人们已经研究了可溶性CD40配体(sCD40L)或klotho基因在肺癌细胞中的作用，但对于klotho与sCD40L联合在肺癌中的功能知之甚少。本研究旨在探讨klotho联合sCD40L对A549肺癌细胞系（CD40阳性）的生物学效应及其可能机制。选择肺癌A549细胞作为靶细胞，并通过可溶性CD40配体（sCD40L）刺激CD40信号。本研究发现klotho、可溶性CD40配体及其组合可通过抑制细胞周期、上调Bax基因表达和（或）下调Bcl-2基因表达来增加A549细胞的细胞增殖抑制和凋亡率；且两者联用对A549细胞凋亡和增殖抑制作用较单独使用klotho或sCD40L更强。这些数据表明klotho和sCD40L的组合可能提供治疗非小细胞肺癌的有效方法。
Although the roles of soluble CD40 ligand (sCD40L) or the klotho gene in lung cancer cells have been studied, little is known about the functions of klotho combined with sCD40L in lung cancer. The present study was designed to investigate the biological effects of klotho combined with sCD40L on the A549 lung cancer cell line (CD40 positive) and their possible mechanisms. Lung cancer A549 cells were chosen as target cells and CD40 signals were stimulated by soluble CD40 ligand (sCD40L). In this study, we found that klotho, soluble CD40 ligand and their combination can increase cell proliferation inhibition and the apoptosis rate in A549 cells by inhibiting the cell cycle, up-regulating Bax gene expression and (or) down-regulating Bcl-2 gene expression; and their combination has a stronger effect on A549 cell apoptosis and proliferation inhibition compared to klotho or sCD40L alone. These data suggest that the combination of klotho and sCD40L may provide an efficient method to treat non-small cell lung cancer.