Short chain fatty acids but not lactate or succinate stimulate mucus release in the rat colon
Short chain fatty acids but not lactate or succinate stimulate mucus release in the rat colon
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DOI：
10.1016/s1095-6433(00)00183-5
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发表时间：
2000-04-01
影响因子：
2.3
通讯作者：
Sakata, T
中科院分区：
文献类型：
--
作者：
Shimotoyodome, A;Meguro, S;Sakata, T
背景：短链脂肪酸(SCFA)影响多种肠道功能。粘液是肠粘膜屏障的重要生理成分。然而，SCFAs或其他有机酸对肠道粘液释放的影响还知之甚少。本研究的目的是探讨管腔SCFA是否刺激粘液释放进入大鼠结肠。方法：SCFA溶液。将乳酸或琥珀酸注入麻醉大鼠的结肠，然后测定流出物中己糖的含量。我们还观察了胆碱能拮抗剂对SCFA作用的影响。结果：SCFA混合物(75 mM醋酸盐、35 mM丙酸盐和20 mM丁酸盐)或单独的SCFA(130 MM)以类似的方式增加粘液释放到结肠。单独的单链脂肪酸，而不是乳酸或琥珀酸，以类似的浓度依赖方式刺激结肠粘液的分泌。丁酸盐在20 mM时对结肠粘液分泌有刺激作用，而醋酸盐、丙酸、乳酸和琥珀酸在该浓度下对结肠粘液分泌无刺激作用。用抗胆碱能药预处理可减弱SCFAs对粘液分泌的刺激作用。结论：腔内单链脂肪酸可通过胆碱能神经机制促进大鼠结肠粘液释放，而不是乳酸或琥珀酸。(C)2000 Elsevier Science Inc.保留所有权利。
Background: Short chain fatty acids (SCFAs) affect various intestinal functions. Mucus is an important physiological component of the intestinal mucosal barrier. However, the effect of SCFAs or other organic acids on the intestinal mucus release is poorly understood. The aim of this study was to investigate whether lumen SCFA stimulates mucus release into the rat colon. Methods: A solution of SCFA. lactate or succinate was-infused into the colon of anesthetized rats, and we then measured the hexose content of the effluent. We also examined the influence of cholinergic antagonists on the effects of SCFA. Results: A SCFA mixture (75 mM acetate, 35 mM propionate and 20 mM butyrate) or individual SCFAs (130 mM) increased the mucus release into the colon in a similar manner. The individual SCFAs, but not lactate or succinate, stimulated colonic mucus secretion in similar concentration-dependent manners. Butyrate stimulated colonic mucus secretion at 20 mM, but acetate, propionate, lactate and succinate at this concentration did not. Pretreatment with an anti-cholinergic agent diminished the stimulatory effects of SCFAs on mucus secretion. Conclusions: Lumen SCFAs, but not lactate or succinate, stimulate mucus release from the rat colon via a cholinergic nerve mechanism. (C) 2000 Elsevier Science Inc. All rights reserved.