The effects of Streptomyces hyaluronidase on tissue organization and cell cycle time in rat embryos.
The effects of Streptomyces hyaluronidase on tissue organization and cell cycle time in rat embryos.
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链霉菌透明质酸酶对大鼠胚胎组织组织和细胞周期时间的影响。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
M. Solursh
中科院分区：
文献类型：
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作者：
G. Morriss;F. Tuckett;M. Solursh
将第9天的大鼠胚胎（具有颅神经板或极早期神经褶皱的后期前体阶段）在含有20 TRU ml-1链霉菌透明质酸酶的培养基中培养6-48 h的不同时间段。暴露于酶导致间充质细胞外基质的大量减少。通过阿辛蓝染色证实酶进入胚胎，这表明细胞外和细胞表面透明质酸盐大量减少。颅神经元发育迟缓，但不受抑制，神经嵴和初级间充质细胞的迁移发生。一般而言，48 h时形态正常。主要影响是生长：胚胎较小，神经上皮细胞数量略有减少，间充质细胞数量大大减少。神经上皮细胞周期时间略有延长，间充质细胞周期时间延长一倍以上。对这两种组织生长速率的差异效应反映了透明质酸盐的正常分布，其在间充质细胞外基质中特别丰富。
Day 9 rat embryos (late presomite stage with cranial neural plate or very early neural folds) were cultured for various periods of time from 6-48 h in medium containing 20 TRU ml-1 Streptomyces hyaluronidase. Exposure to the enzyme resulted in considerable reduction of mesenchymal extracellular matrix. Access of the enzyme to the embryo was confirmed by alcian blue staining which indicated considerable reduction of extracellular and cell surface hyaluronate. Cranial neurulation was retarded, but not inhibited, and migration of both neural crest and primary mesenchyme cells occurred. In general, morphology was normal at 48 h. The major effect was on growth: embryos were smaller, with slightly reduced neuroepithelial cell number and greatly reduced mesenchymal cell number. Neuroepithelial cell cycle time was slightly prolonged, and that of the mesenchyme more than doubled. This differential effect on the growth rates of these two tissues reflects the normal distribution of hyaluronate, which is particularly abundant in the mesenchymal extracellular matrix.