Carbonic Anhydrase of Chlamydomonas: Purification and Studies on its Induction Using Antiserum against Chlamydomonas Carbonic Anhydrase
Carbonic Anhydrase of Chlamydomonas: Purification and Studies on its Induction Using Antiserum against Chlamydomonas Carbonic Anhydrase
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衣藻碳酸酐酶的纯化及其抗衣藻碳酸酐酶抗血清的诱导研究
DOI：
10.1093/oxfordjournals.pcp.a076892
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发表时间：
1985
影响因子：
4.9
通讯作者：
S. Miyachi
中科院分区：
文献类型：
--
作者：
Shi;M. Tsuzuki;S. Miyachi
碳酸酐酶（EC 4.2.1.1; CA）是用亲和层析法从普通空气中光自养生长的单细胞绿色莱茵衣藻（Chlamydiumreinhardti）细胞中纯化出来的。用此纯化CA制备的兔抗血清与衣原体CA发生交叉反应，但与菠菜叶CA和牛红细胞CA均不发生交叉反应。当向藻细胞提供的CO2浓度从4%降低到正常空气水平（0.04%）时，CA活性和CA蛋白含量（用免疫扩散法测定）呈平行上升趋势。相反，当CO2浓度从空气水平升高到4%CO2时，CA活性及其以培养体积表示的含量保持相当恒定。这些结果表明，CA蛋白的合成诱导时，CO2浓度从4到0.04%的藻类生长过程中降低。另一方面，当CO2浓度从空气水平升高到4%时，CA的合成停止。
Carbonic anhydrase (EC 4.2.1.1; CA) was purified by affinity chromatography from cells of the unicellular green algaChlamydomonas reinhardtiiwhich had been grown photoautotrophically in ordinary air. Antiserum raised in rabbit against this purified CA crossreacted withChlamydomonasCA but not with spinach leaf CA nor bovine erythrocyte CA. When the CO2concentration provided to the algal cells was decreased from 4% to the ordinary air level (0.04%), CA activity and the content of CA protein determined by the immunodiffusion test showed parallel increases. In contrast, when the CO2concentration was raised from air level to 4% CO2CA activity and its content expressed on the basis of culture volume remained rather constant. These results indicate that synthesis of the CA protein is induced when the CO2concentration is lowered from 4 to 0.04% during algal growth. On the other hand, the synthesis of CA stops when CO2concentration is raised from air level to 4%.