A MORE SPECIFIC REAGENT FOR THE DETERMINATION OF SUGAR IN URINE

A MORE SPECIFIC REAGENT FOR THE DETERMINATION OF SUGAR IN URINE
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用于测定尿液中糖分的更具特异性的试剂

DOI:
10.1016/s0021-9258(18)84848-7
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发表时间:
1925
影响因子:
4.8
通讯作者:
J. Sumner
J. Sumner
中科院分区:
生物学2区
文献类型:
--
作者:
J. Sumner

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这种新试剂在测定正常人和糖尿病患者尿液中的糖方面都是完全令人满意的。它与正常尿液呈强烈的颜色,完全符合标准,并且6小时内值不变。所获得的数字比上述试剂所给出的数字低40%以上。即使在使用木炭之后,这也比Benedict和Osterberg方法给出的值低,并且似乎可以肯定的是,新试剂几乎完全特异于还原糖。根据贝内的说明,用活性炭振摇尿液后-
The new reagent is entirely satisfactory in every respect for the estimation of sugar both in normal and in diabetic urine. It gives an intense color with normal urine which matches the standards perfectly and which does not change in value for 6 hour. The figures obtained are over 40 per cent lower than those given by the previously described reagent. This is a lower value than is given by the Benedict and Osterberg method even after using charcoal, and it seems certain that the new reagent is almost completely specific for reducing sugars. After shaking urine with charcoal according to the directions of Bene-