Immunodetection of 11β-Hydroxysteroid Dehydrogenase Type 2 in Human Mineralocorticoid Target Tissues: Evidence for Nuclear Localization.
Immunodetection of 11β-Hydroxysteroid Dehydrogenase Type 2 in Human Mineralocorticoid Target Tissues: Evidence for Nuclear Localization.
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DOI：
10.1210/endo.138.3.4994
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发表时间：
1997-03
期刊：
影响因子：
4.8
通讯作者：
M. Shimojo;M. Ricketts;M. Petrelli;P. Moradi;G. Johnson;A. Bradwell;M. Hewison;A. Howie;P. Stewart
中科院分区：
文献类型：
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作者：
M. Shimojo;M. Ricketts;M. Petrelli;P. Moradi;G. Johnson;A. Bradwell;M. Hewison;A. Howie;P. Stewart
11β-羟基类固醇脱氢酶（11βHSD）是一种负责将具有生理活性的皮质醇转化为无活性的可的松的酶复合物;该酶的两种异构体已被克隆和表征。最近根据人11β HSD 2基因突变解释的高血压综合征患者的临床观察表明，11β HSD 2亚型在决定盐皮质激素受体（MR）的特异性方面起着至关重要的作用。我们用合成的多抗原肽在绵羊体内制备了抗人11β HSD 2的新抗体，并检测了11β HSD 2在盐皮质激素靶组织中的定位和亚细胞分布。免疫纯化的抗体识别胎盘，滋养层和远端结肠中约44 kDa的单一条带。在肾组织中，始终观察到约44和48 kDa的两条条带。在蜕膜、肾上腺或肝脏中未观察到信号。免疫过氧化物酶研究在…
11β-Hydroxysteroid dehydrogenase (11βHSD) is an enzyme complex responsible for the conversion of hormonally active cortisol to inactive cortisone; two isoforms of the enzyme have been cloned and characterized. Clinical observations from patients with the hypertensive syndrome apparent mineralocorticoid excess, recently explained on the basis of mutations in the human 11βHSD2 gene, suggest that it is the 11βHSD2 isoform that serves a vital role in dictating specificity upon the mineralocorticoid receptor (MR). We have raised a novel antibody in sheep against human 11βHSD2 using synthetic multiantigenic peptides and have examined the localization and subcellular distribution of 11βHSD2 in mineralocorticoid target tissues. The immunopurified antibody recognized a single band of approximately 44 kDa in placenta, trophoblast, and distal colon. In kidney tissue, two bands of approximately 44 and 48 kDa were consistently observed. No signal was seen in decidua, adrenal, or liver. Immunoperoxidase studies on the ...