Profile of rhythmic gene expression in the livers of obese diabetic KK-Ay mice
Profile of rhythmic gene expression in the livers of obese diabetic KK-Ay mice
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DOI：
10.1016/j.bbrc.2006.06.044
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发表时间：
2006-08-11
影响因子：
3.1
通讯作者：
Fujimura, Akio
中科院分区：
文献类型：
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作者：
Ando, Hitoshi;Oshima, Yasuo;Fujimura, Akio
尽管在包括肝脏在内的大多数组织中表达的许多基因表现出昼夜节律调节，但通常仅在每天的一个预定时间检查基因表达谱。在这项研究中，我们研究了肥胖糖尿病在 24 小时内以 6 小时为间隔对各种基因的肝脏 mRNA 水平的影响。微阵列分析显示，许多基因不仅在对照 KK 小鼠中而且在肥胖糖尿病 KK-A(y) 小鼠中都有节律地表达。实时定量 PCR 验证了两种菌株中 23 个假定的昼夜节律表达基因中的 19 个显示出显着的 24 小时节律性。然而，肥胖糖尿病减弱了 19 个基因中 10 个基因的表达节律。更重要的是，全天仅在两个基因中观察到肥胖糖尿病的影响。这些结果表明观察时间影响昼夜节律表达基因的基因表达分析结果。 (c) 2006 Elsevier Inc. 保留所有权利。
Although a number of genes expressed in most tissues, including the liver, exhibit circadian regulation, gene expression profiles are usually examined only at one scheduled time each day. In this study, we investigated the effects of obese diabetes on the hepatic mRNA levels of various genes at 6-h intervals over a single 24-h period. Microarray analysis revealed that many genes are expressed rhythmically, not only in control KK mice but also in obese diabetic KK-A(y) mice. Real-time quantitative PCR verified that 19 of 23 putative circadianly expressed genes showed significant 24-h rhythmicity in both strains. However, obese diabetes attenuated these expression rhythms in 10 of 19 genes. More importantly, the effects of obese diabetes were observed throughout the day in only two genes. These results suggest that observation time influences the results of gene expression analyses of genes expressed circadianly. (c) 2006 Elsevier Inc. All rights reserved.