Genetic differentiation and history of populations of the Italian treefrog Hyla intermedia:: lack of concordance between mitochondrial and nuclear markers
Genetic differentiation and history of populations of the Italian treefrog Hyla intermedia:: lack of concordance between mitochondrial and nuclear markers
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DOI：
10.1007/s10709-006-9102-9
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发表时间：
2007-07-01
期刊：
影响因子：
1.5
通讯作者：
Nascetti, Giuseppe
中科院分区：
文献类型：
--
作者：
Canestrelli, Daniele;Verardi, Andrea;Nascetti, Giuseppe
采用双亲本遗传标记（23个等位酶位点）和母本遗传标记（部分线粒体细胞色素b基因）研究了意大利树蛙（Hyla intermedia） 33个居群的遗传分化。两个标记都证明了两个主要的人口群体，分别位于亚平宁山脉北部和南部。然而，这两组之间在同酶上的分化模式远不如在mtDNA上的分化模式明显，导致线粒体水平的基因流估计比核水平低25倍。此外，两组间的mtDNA差异在无脊椎两栖动物的两个共特异谱系中尤为显著（p -距离平均为9.04%），而它们在同酶上的平均遗传距离相对较低（D-NEI = 0.07）。这种核与线粒体遗传变异的对比模式在以下背景下进行了讨论：(1)标记特异性选择；(2)二次接触和性别偏向基因流；(3)祖先多态性和从北向南的定植。最后，我们强调在群体遗传研究中，使用具有不同进化特性的多个标记可以帮助揭示更复杂进化历史的存在。
The genetic differentiation among 33 populations of the Italian treefrog, Hyla intermedia (Anura: Hylidae), was investigated using both biparentally (23 allozyme loci) and maternally (partial mitochondrial cytochrome b gene) inherited markers. Two main population groups were evidenced by both markers, located north and south of the northern Apennines. However, the pattern of differentiation between these two groups was much less pronounced at allozymes than at mtDNA, leading to gene flow estimates that were 25 times lower at mitochondrial than at nuclear level. Also, the mtDNA divergence between the two groups was particularly marked for two cospecific lineages of anuran amphibians (the P-distance being on average 9.04%), while their average genetic distance at allozymes was comparatively low (D-NEI = 0.07). This contrasting pattern of nuclear versus mitochondrial genetic variation is discussed in the context of: (1) marker specific selection, (2) secondary contact and sex-biased gene flow and (3) ancestral polymorphism and colonization from north to south. Finally we emphasize how, for population genetic studies, the use of multiple markers having distinct evolutionary properties can help unravel the existence of more complex evolutionary histories.