Yellowing disease of tomatoes caused by Tomato infectious chlorosis virus newly recognized in Japan
Yellowing disease of tomatoes caused by Tomato infectious chlorosis virus newly recognized in Japan
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DOI：
10.1007/s10327-002-0015-x
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发表时间：
2003-02
影响因子：
1.2
通讯作者：
S. Hartono;T. Natsuaki;H. Sayama;H. Atarashi;S. Okuda
中科院分区：
文献类型：
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作者：
S. Hartono;T. Natsuaki;H. Sayama;H. Atarashi;S. Okuda
2001年，在日本群马县和栃木县的温室和大田种植的番茄植株（Lycopersicon esculentumMiller）上观察到黄化、叶脉间失绿、卷叶和坏死斑点等病毒样症状。病原体的特征，如颗粒形态、粉虱传播和症状学，与番茄传染性褪绿病毒（TICV）和番茄褪绿病毒（长线病毒科病毒属）的特征相似。提取的双链 RNA 的电泳分析揭示了两个大约 7 kbp 的主要物种。使用长线病毒热休克蛋白70同源物和差异外壳蛋白基因的特异性引物对逆转录聚合酶链反应产物进行测序，表明该病毒是TICV。
In 2001 virus-like symptoms of yellowing, interveinal chlorosis, leaf-rolling, and necrotic fleck were observed on greenhouse- and field-grown tomato plants (Lycopersicon esculentumMiller) in Gunma and Tochigi Prefectures, Japan. Characteristics of the causal agent, such as particle morphology, whitefly transmission, and symptomatology, are similar to those ofTomato infectious chlorosis virus(TICV) andTomato chlorosis virus, species of the genusCrinivirusin the familyClosteroviridae. Electrophoretic analysis of extracted double-stranded RNAs revealed two major species of approximately 7 kbp. Sequencing of reverse transcription-polymerase chain reaction products using the specific primers for heat shock protein 70 homolog and diverged coat protein genes of closteroviruses indicated that the virus was TICV.