Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP)
Repair kinetics of abasic sites in mammalian cells selectively monitored by the aldehyde reactive probe (ARP)
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DOI：
10.1080/07328319808005194
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Kubo, K
中科院分区：
文献类型：
--
作者：
Asaeda, A;Ide, H;Kubo, K
人甲基嘌呤N-糖基化酶（MPG）的活性进行了研究，通过监测脱碱基（AP）的网站所产生的烷基化碱的去除。在MMS处理的HeLa细胞中，AP位点的数量在3 h时短暂增加，然后逐渐下降至24 h时的40%。腺嘌呤，AP核酸内切酶的抑制剂，在MMS处理的细胞的修复孵育中的存在下诱导AP位点的中度积累，这表明腺嘌呤代谢产物抑制MPG以及AP核酸内切酶的活性。
Human methylpurine N-glycosylase (MPG) activity was investigated by monitoring abasic (AP) sites resulting from removal of alkylated bases. The amount of AP sites in MMS-treated HeLa cells transiently increased at 3 h, then gradually decreased to 40% at 24 h. The presence of adenine, an inhibitor of AP endonucleases, in the repair incubation of MMS-treated cells induced moderate accumulation of AP sites, suggesting inhibition of the activities of MPG as well as AP endonucleases by adenine metabolites.