Quantitative 129Xe MRI detects early impairment of gas-exchange in a rat model of pulmonary hypertension.
Quantitative 129Xe MRI detects early impairment of gas-exchange in a rat model of pulmonary hypertension.
复制标题
定量 129Xe MRI 可检测肺动脉高压大鼠模型中气体交换的早期损伤。
DOI：
10.1038/s41598-020-64361-1
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发表时间：
2020
影响因子：
4.6
通讯作者：
Driehuys,Bastiaan
中科院分区：
文献类型：
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作者：
Virgincar,RohanS;Nouls,JohnC;Wang,Ziyi;Degan,Simone;Qi,Yi;Xiong,Xinyu;Rajagopal,Sudarshan;Driehuys,Bastiaan
超极化129磁共振成像（MRI）能够对肺部气体交换进行区域映射，并已在人类和动物模型类似物的广泛肺部疾病中得到应用。本研究首次将129 SrDNA MRI应用于野百合碱肺动脉高压大鼠模型。临床前肺动脉高压（一种导致右心衰竭和死亡的肺血管系统疾病）的此类模型通常采用侵入性程序（如右心导管插入术和组织病理学）进行评估。本文的工作改编自临床129氚MRI的方案，以实现在Bruker 7 T扫描仪上对肺动脉高压大鼠模型的临床前成像。129氚光谱和气体交换成像显示，在疾病进展的早期，红细胞对129氚的摄取减少，在稍后的时间点，伴随着屏障组织对129氚的摄取增加，水肿，和通风缺陷-所有这些都是野百合碱模型的显著特征。成像结果通过H&E组织学进行验证，其显示小动脉重塑的证据。这项概念验证研究表明，超极化129 MRI具有很强的潜力，可用于无创监测临床前模型中肺动脉高压的进展，并可能评估对治疗的反应。
Hyperpolarized129Xe magnetic resonance imaging (MRI) is capable of regional mapping of pulmonary gas-exchange and has found application in a wide range of pulmonary disorders in humans and animal model analogs. This study is the first application of129Xe MRI to the monocrotaline rat model of pulmonary hypertension. Such models of preclinical pulmonary hypertension, a disease of the pulmonary vasculature that results in right heart failure and death, are usually assessed with invasive procedures such as right heart catheterization and histopathology. The work here adapted from protocols from clinical129Xe MRI to enable preclinical imaging of rat models of pulmonary hypertension on a Bruker 7 T scanner.129Xe spectroscopy and gas-exchange imaging showed reduced129Xe uptake by red blood cells early in the progression of the disease, and at a later time point was accompanied by increased uptake by barrier tissues, edema, and ventilation defects—all of which are salient characteristics of the monocrotaline model. Imaging results were validated by H&E histology, which showed evidence of remodeling of arterioles. This proof-of-concept study has demonstrated that hyperpolarized129Xe MRI has strong potential to be used to non-invasively monitor the progression of pulmonary hypertension in preclinical models and potentially to also assess response to therapy.