OXYGEN-INDUCED RETINOPATHY IN THE RAT - POSSIBLE CONTRIBUTION OF PEROXIDATION REACTIONS
OXYGEN-INDUCED RETINOPATHY IN THE RAT - POSSIBLE CONTRIBUTION OF PEROXIDATION REACTIONS
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DOI：
10.1007/bf02482607
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发表时间：
1990-03-01
影响因子：
1.4
通讯作者：
PENN, JS
中科院分区：
文献类型：
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作者：
PENN, JS
白化病大鼠从出生到14日龄一直保持在60%的大气氧中。同时在室内空气中饲养与之匹配的对照动物。部分大鼠在处死和视网膜解剖前用印度墨水灌注，以研究氧饲养对视网膜血管的影响。通过这种方法，发现氧气饲养的动物持续36%的视网膜血管损失。处死后立即取出其他动物的视网膜，并通过以下三种方法之一检查脂质过氧化的证据：1）测定脂质过氧化产物的存在，2）测定多不饱和脂肪酸的损失，3）测定视网膜维生素E水平。这些测定结果均表明，在氧饲养大鼠的视网膜中发生了过氧化反应。通过母鼠的饮食补充幼鼠的视网膜维生素E。这种治疗导致视网膜维生素E的水平比喂食大鼠食物的母亲的幼崽增加两倍。氧气饲养的维生素E补充大鼠持续显着减少闭塞的血管比非补充氧气饲养的动物。
Albino rats were maintained in 60% atmospheric oxygen from birth through 14 days of age. Age-matched controls were simultaneously raised in room air. Some rats were perfused with India ink before sacrifice and retinal dissection order to study the effect of oxygen-rearing on the retinal vasculature. By this method it was found that oxygen-reared animals sustained a 36% loss of retinal blood vessels. Other animals'' retinas were removed immediately after sacrifice and examined for evidence of lipid peroxidation by one of three means: 1) a determination of the presence of products of lipid peroxidation, 2) a measure of the loss polyunsaturated fatty acids, and 3) a determination of retinal vitamin E level. Each of these determinations indicated that peroxidation reactions had occurred in the retinas of oxygen-reared rats. Retinal vitamin E was supplemented in the young rats through the diet of the mothers. This treatment resulted in a two-fold increase of retinal vitamin E over levels in pups of mothers fed rat chow. Oxygen-reared vitamin E-supplemented rats sustained significantly less obliteration of blood vessels than non-supplemented oxygen-reared animals.