Identification of Mycobacterium avium genes up-regulated in cultured macrophages and in mice
Identification of Mycobacterium avium genes up-regulated in cultured macrophages and in mice
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DOI：
10.1016/j.femsle.2004.08.014
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发表时间：
2004-10-01
影响因子：
2.1
通讯作者：
Bermudez, LE
中科院分区：
文献类型：
--
作者：
Danelishvili, L;Poort, MJ;Bermudez, LE
为了研究鸟分枝杆菌感染巨噬细胞后的基因表达，我们建立了一个M。在耻垢分枝杆菌中编码绿色荧光蛋白（GFP）的无启动子基因上游的禽启动子文库。在感染U937巨噬细胞后，评价克隆的GFP表达增加。一些M。在巨噬细胞感染后24和48小时，禽流感基因上调超过3倍。M. M. avium表达的基因。巨噬细胞中生长过程中的smeglobulin包括编码转运/结合蛋白、大分子合成、修饰和降解的基因，以及目前尚不知道其功能的绝大多数基因。对于一些未知基因，在细菌如麻风分枝杆菌、鼠伤寒沙门氏菌和根癌农杆菌中鉴定了同源物。在体外和体内巨噬细胞感染期间，使用真实的时间RT-PCR定量所选基因的转录物。对M.在M.体外和小鼠巨噬细胞感染24 h后，(C)2004年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
To investigate Mycobacterium avium gene expression upon infection of macrophages, we created a M. avium-promoter library upstream of a promoter-less gene encoding the green fluorescent protein (GFP) in Mycobacterium smegmatis. Clones were evaluated for increased expression of GFP after infection of U937 macrophages. A number of M. avium genes were up-regulated more than 3-fold after 24 and 48 h following macrophage infection. M. avium genes expressed by M. smegmatis during growth in macrophages include genes encoding transport/binding proteins, synthesis, modification and degradation of macromolecules, and a great majority of genes for which no function is currently known. For some of the unknown genes, homologues were identified in bacteria such as Mycobacterium leprae, Salmonella typhimurium and Agrobacterium tumefaciens.In order to investigate if these genes were also expressed in M. avium during macrophage infection in vitro and in vivo, transcripts of selected genes were quantified using real time RT-PCR. Evaluation of most expressed genes in M. smegmatis confirmed their upregulation in M. avium after 24 h infection of macrophages in vitro and mice. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.