PERIVITELLINE SPACE OF MAMMALIAN OOCYTES - EXTRACELLULAR-MATRIX OF UNFERTILIZED OOCYTES AND FORMATION OF A CORTICAL GRANULE ENVELOPE FOLLOWING FERTILIZATION
PERIVITELLINE SPACE OF MAMMALIAN OOCYTES - EXTRACELLULAR-MATRIX OF UNFERTILIZED OOCYTES AND FORMATION OF A CORTICAL GRANULE ENVELOPE FOLLOWING FERTILIZATION
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DOI：
10.1002/mrd.1080310208
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发表时间：
1992-02-01
影响因子：
2.5
通讯作者：
TALBOT, P
中科院分区：
文献类型：
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作者：
DANDEKAR, P;TALBOT, P
细胞外基质（ECM）周围未受精和受精的哺乳动物卵母细胞进行了研究超微结构中的钌红的存在下制备的样品，以促进细胞外物质的稳定。未受精的小鼠、仓鼠和人卵母细胞在其卵周隙（PVS）中具有包含颗粒和细丝的ECM。这种基质在人类中比在仓鼠和小鼠中更丰富。颗粒/细丝基质似乎与钌红处理后在卵丘和辐射冠细胞之间观察到的基质相同，并且先前显示包含蛋白质和透明质酸。通过在固定过程中加入钌红，可以证明仓鼠、小鼠和人类受精卵母细胞的PVS中存在皮质颗粒渗出物。大部分皮质颗粒渗出物被捕获在PVS中，并在受精卵母细胞周围形成新的被膜。当用钌红染色时，这种物质是颗粒状的，并且似乎均匀地分散在整个卵母细胞表面周围。我们把这种新的被膜称为皮质颗粒被膜。在所有三个物种的所有发育阶段（包括囊胚）的PVS中均观察到这种包膜。小鼠和仓鼠囊胚孵化后，皮质颗粒包膜不再存在。讨论了该信封的可能功能。
Extracellular matrices (ECM) present around unfertilized and fertilized mammalian oocytes were studied ultrastructurally in samples prepared in the presence of ruthenium red to facilitate stabilization of extracellular materials. Unfertilized mouse, hamster, and human oocytes have an ECM comprising granules and filaments in their perivitelline spaces (PVS). This matrix is more abundant in the human than in hamsters and mice. The granule/filament matrix appears identical to the matrix seen between cumulus and corona radiata cells following ruthenium red processing and previously shown to comprise protein and hyaluronic acid. By including ruthenium red during fixation, it is possible to demonstrate the existence of cortical granule exudate in the PVS of fertilized oocytes from hamsters, mice, and humans. Much of the cortical granule exudate is trapped in the PVS and forms a new coat around the fertilized oocyte. This material is particulate when stained with ruthenium red and appears to be uniformly dispersed around the entire oocyte surface. We refer to this new coat as the cortical granule envelope. This envelope is observed in the PVS of all developmental stages up to and including blastocysts in all three species. Following hatching of mouse and hamster blastocysts, the cortical granule envelope is no longer present. Possible functions of this envelope are discussed.