Comparative Genome Analysis of Mycobacterium avium Revealed Genetic Diversity in Strains that Cause Pulmonary and Disseminated Disease
Comparative Genome Analysis of Mycobacterium avium Revealed Genetic Diversity in Strains that Cause Pulmonary and Disseminated Disease
复制标题
鸟分枝杆菌的比较基因组分析揭示了引起肺部和播散性疾病的菌株的遗传多样性
作者：
K. Uchiya;Hiroyasu Takahashi;T. Yagi;M. Moriyama;T. Inagaki;K. Ichikawa;T. Nakagawa;T. Nikai;K. Ogawa
鸟分枝杆菌复合体（MAC）感染在免疫功能低下的宿主（例如人类免疫缺陷病毒（HIV）阳性患者）中引起播散性疾病，并且在没有全身免疫抑制的人中引起肺部疾病，这在许多国家一直在增加。在日本，由M. avium引起的感染比M.胞内的探讨影响M. avium，我们测定了以前未报道的M.鸟亚种从一个HIV阴性的肺MAC病患者中分离的hominissuis菌株TH 135，并将其与已知的M.来源于患有MAC疾病的获得性免疫缺陷综合征患者的禽流感病毒株104。菌株TH 135的基因组由具有4，636个编码序列的4，951，217-bp环状染色体组成。比较分析发现，两株菌共有4,012个基因，菌株TH 135和104分别有624和1,108个独特基因。在两株菌的基因组中均发现了包括毒力相关基因在内的许多株系特异性区域，除个别区域外，特异性区域的G+C含量均低于相应染色体的平均G+C含量。对临床分离株进行病毒特异性区域基因筛查发现，肺MAC病患者分离株中TH 135株特异性基因的检出率相对较高，而HIV阳性患者分离株中104株特异性基因的检出率相对较高。总的来说，M。导致肺部和播散性疾病的禽流感病毒株具有独特的遗传特征，这表明在病毒株进化过程中获得特定基因在MAC疾病的病理表现中发挥了重要作用。
Mycobacterium avium complex (MAC) infection causes disseminated disease in immunocompromised hosts, such as human immunodeficiency virus (HIV)-positive patients, and pulmonary disease in persons without systemic immunosuppression, which has been increasing in many countries. In Japan, the incidence of pulmonary MAC disease caused by M. avium is about 7 times higher than that caused by M. intracellulare. To explore the bacterial factors that affect the pathological state of MAC disease caused by M. avium, we determined the complete genome sequence of the previously unreported M. avium subsp. hominissuis strain TH135 isolated from a HIV-negative patient with pulmonary MAC disease and compared it with the known genomic sequence of M. avium strain 104 derived from an acquired immunodeficiency syndrome patient with MAC disease. The genome of strain TH135 consists of a 4,951,217-bp circular chromosome with 4,636 coding sequences. Comparative analysis revealed that 4,012 genes are shared between the two strains, and strains TH135 and 104 have 624 and 1,108 unique genes, respectively. Many strain-specific regions including virulence-associated genes were found in genomes of both strains, and except for some regions, the G+C content in the specific regions was low compared with the mean G+C content of the corresponding chromosome. Screening of clinical isolates for genes located in the strain-specific regions revealed that the detection rates of strain TH135-specific genes were relatively high in specimens isolated from pulmonary MAC disease patients, while, those of strain 104-specific genes were relatively high in those from HIV-positive patients. Collectively, M. avium strains that cause pulmonary and disseminated disease possess genetically distinct features, and it suggests that the acquisition of specific genes during strain evolution has played an important role in the pathological manifestations of MAC disease.
DOI：
10.1073/pnas.92.11.5189
发表时间：
1995-05-23
影响因子：
11.1
作者：
GOBIN, J;MOORE, CH;HORWITZ, MA
通讯作者：
HORWITZ, MA
影响因子：
7
作者：
Darling, ACE;Mau, B;Perna, NT
通讯作者：
Perna, NT
影响因子：
3.2
作者：
Song, Houhui;Sandie, Reatha;Niederweis, Michael
通讯作者：
Niederweis, Michael