In vitro evaluation of the anti-proteolytic and cross-linking effect of mussel-inspired monomer on the demineralized dentin matrix
In vitro evaluation of the anti-proteolytic and cross-linking effect of mussel-inspired monomer on the demineralized dentin matrix
复制标题
贻贝单体对脱矿牙本质基质的抗蛋白水解和交联作用的体外评价。
DOI：
10.1016/j.jdent.2021.103720
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发表时间：
2021-06-17
影响因子：
4.4
通讯作者：
Yiu, Cynthia Kar Yung
中科院分区：
文献类型：
--
作者：
Li, Kang;Zhang, Zhongni;Yiu, Cynthia Kar Yung
目的：目的观察N-（3，4-二羟基苯乙基）甲基丙烯酰胺（DMA）对脱矿牙本质基质的抗蛋白水解和交联作用。研究方法：选择四种实验溶液：50%乙醇/水溶液（对照）; 1、5和10 mmol/L DMA溶于50%乙醇/水溶液。将健康人磨牙切片，制备尺寸为1 × 1 × 6 mm的牙本质梁。牙本质梁用10%磷酸脱矿8 h以去除磷灰石。将脱矿标本随机分为4组，分别在4种实验溶液中浸泡1 h。处理后，通过在0.1 mg/mL胶原酶溶液中储存处理的标本24 h，评估极限拉伸强度（UTS）、干质量损失和羟脯氨酸释放。对每组另外10个样本的膨胀率进行评价。用场发射扫描电镜观察DMA与牙本质基质的相互作用。UTS数据通过双因素ANOVA分析，然后进行Tukey检验，其他数据通过单因素ANOVA分析，然后进行Tukey检验（α = 0.05）。结果如下：双因素方差分析因子、不同溶液（p <0.001）、胶原酶降解（p <0.001）及其相互作用（p <0.001）均显著影响UTS。10 mM DMA处理组的牙本质干重损失率、羟脯氨酸释放率和脱矿牙本质基质溶胀率均显著低于其他处理组（p <0.05）。FESEM观察显示，随着DMA浓度的增加，牙本质基质的结构得到保护，牙本质胶原网络的孔隙率降低。结论：用10 mM DMA/乙醇溶液处理1 h能够增强脱矿牙本质基质对胶原酶降解的机械性能，并可能在临床上用于改善混合层的耐久性。临床意义：10 mM DMA/乙醇引物可为牙科医生提供另一种选择，以增强脱矿牙本质基质的机械性能并抵抗胶原酶对其的降解。
Objectives: To evaluate the anti-proteolytic and cross-linking effect of N-(3,4-dihydroxyphenethyl)meth-acrylamide (DMA) on the demineralized dentin matrix in vitro. Methods: Four experimental solutions were selected: 50% ethanol/water solution (Control); 1, 5, and 10 mmol/L DMA dissolved in 50% ethanol/water solution. Sound human molars were sectioned to produce dentin beams with dimension of 1x1x6 mm. The dentin beams were demineralized with 10% phosphoric acid for 8 h to remove the apatite. The demineralized specimens were randomly separated into four groups and immersed in the four experimental solutions for 1 h. After the treatment, the ultimate tensile strength (UTS), loss of dry mass and the release of hydroxyproline by storing the treated specimens in 0.1 mg/mL collagenase solution for 24 h were assessed. The swelling ratio of another ten specimens from each group were evaluated. The interaction between DMA with dentin matrix was observed under Field Emission Scanning Electron Microscopy (FESEM). UTS data was analyzed by two-way ANOVA followed by Tukey test, and the other data was analyzed by one-way ANOVA followed by Tukey test (alpha = 0.05). Results: The two-way ANOVA factors, different solutions (p < 0.001), collagenase degradation (p < 0.001) and their interactions (p < 0.001) all significantly affected the UTS. The 10 mM DMA treatment significantly decreased the percentage of loss of dry mass, release of hydroxyproline and swelling ratio of demineralized dentin matrix compared to other treatment groups (p < 0.05). The FESEM observation depicted that with increasing concentration of DMA, the structure of dentin matrix was protected and the porosity within dentin collagen network was decreased. Conclusions: The treatment by 10 mM DMA/ethanol solution for 1 hour is capable of enhancing the mechanical properties of demineralized dentin matrix against collagenase degradation and may be clinically useful to improve the durability of hybrid layer. Clinical Significance: The 10 mM DMA/ethanol primer may offer an alternative choice for dentists to strengthen the mechanical properties of demineralized dentin matrix and resist its degradation by collagenase.