4-(4'-Methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl bromide: a new electrophoric derivatizing reagent.
4-(4'-Methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl bromide: a new electrophoric derivatizing reagent.
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4-(4-甲基四氟苯基)-2,3,5,6-四氟苄基溴：一种新型电泳衍生试剂。
DOI：
10.1016/j.chroma.2006.09.049
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Giese,RogerW
中科院分区：
文献类型：
--
作者：
Murugaiah,Veeravagu;Naim,Abdul;Peng,Shiqi;Cui,Guohui;Giese,RogerW
将市售的4，4‘-二甲基四氟联苯一步转化为4-(4′-methyltetrafluorophenyl)-2，3，5，6-tetrafluorobenzyl溴化物，以提供一种新的电泳衍生化试剂。当模型分析物2-氟-O6-(2‘-羟乙基)次黄嘌呤与该试剂反应时，产生一种异构体产物的混合物(与其与五氟溴的反应类似，在N7和N9处明显被取代)，并在S/N=10处用气相色谱-电子捕获质谱仪(GC-EC-MS)检测了该混合物的53个光谱图。如预期的那样，MTFB-TFBBR衍生物的挥发度比相应的五氟苯基衍生物小得多(两倍)。预计MTFB-TFBBR有时可用于提供在电泳衍生化/GC-EC-MS分析中遇到较少背景噪声的电泳衍生物。
Commercially-available 4,4′-dimethyloctafluorobiphenyl was converted in a single step to 4-(4′-methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl bromide (MTFP-TFBBr) for the purpose of providing a new electrophoric derivatizing reagent. When reacted with this reagent, 2-fluoro-O6-(2′-hydroxyethyl)hypoxanthine, a model analyte, gave a mixture of isomeric products (apparently substituted at N7 and N9, analogous to its known reaction with pentafluorobenzyl bromide), and 53 femtograms of the mixture was detected at S/N=10 by gas chromatography electron capture mass spectrometry (GC–EC-MS). As intended, the volatility of the MTFB-TFBBr derivative was much less (two-fold) than that of the corresponding pentafluorobenzyl derivative. It is anticipated that MTFB-TFBBr sometimes will be useful in providing an electrophoric derivative that encounters less background noise in analysis by electrophore derivatization/GC–EC-MS.