Functional Improvement of Infarcted Heart by Co-Injection of Embryonic Stem Cells with Temperature-Responsive Chitosan Hydrogel
Functional Improvement of Infarcted Heart by Co-Injection of Embryonic Stem Cells with Temperature-Responsive Chitosan Hydrogel
复制标题
胚胎干细胞与温度响应型壳聚糖水凝胶共注射改善梗塞心脏功能
DOI：
10.1089/ten.tea.2008.0143
复制
发表时间：
2009-06-01
影响因子：
4.1
通讯作者：
Wang, Chang-Yong
中科院分区：
文献类型：
--
作者：
Lu, Wen-Ning;Lue, Shuang-Hong;Wang, Chang-Yong
胚胎干细胞（ESCs）移植治疗心肌梗死可改善心功能。缺血组织中细胞的低保留率和存活率使得细胞移植技术的应用变得困难。在这项研究中，我们使用温度响应的壳聚糖水凝胶（作为支架）与ESCs结合来维持梗死组织中的活细胞。制备具有温度响应性的壳聚糖水凝胶，单独或与小鼠ESCs一起注射到大鼠梗死模型心壁内。结果显示，两组小鼠24小时的细胞保留率和4周的移植物大小均显著高于磷酸盐缓冲盐水对照组。植入4周后，壳聚糖+ ESC组、壳聚糖组和ESC组的心功能、壁厚和梗死区微血管密度均优于PBS对照组。三组中，壳聚糖+ ESC效果最好。本研究结果表明，壳聚糖水凝胶是一种可注射支架，可用于将干细胞输送到梗死心肌。它还可以增加细胞潴留和移植物的大小。心脏功能也保存得很好。
Transplantation of embryonic stem cells (ESCs) can improve cardiac function in treatment of myocardial infarction. The low rate of cell retention and survival within the ischemic tissues makes the application of cell transplantation techniques difficult. In this study, we used a temperature-responsive chitosan hydrogel (as scaffold) combined with ESCs to maintain viable cells in the infarcted tissue. Temperature-responsive chitosan hydrogel was prepared and injected into the infarcted heart wall of rat infarction models alone or together with mouse ESCs. The result showed that the 24-h cell retention and 4 week graft size of both groups was significantly greater than with a phosphate buffered saline control. After 4 weeks of implantation, heart function, wall thickness, and microvessel densities within the infarct area improved in the chitosan + ESC, chitosan, and ESC group more than the PBS control. Of the three groups, the chitosan + ESC performed best. Results of this study indicate that temperature-responsive chitosan hydrogel is an injectable scaffold that can be used to deliver stem cells to infarcted myocardium. It can also increase cell retention and graft size. Cardiac function is well preserved, too.