Histochemical identification of Methicillin-resistant Staphylococcus aureus:: contribution to preventing nosocomial infection
Histochemical identification of Methicillin-resistant Staphylococcus aureus:: contribution to preventing nosocomial infection
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DOI：
10.1053/j.semdp.2007.07.004
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发表时间：
2007-11-01
影响因子：
2.3
通讯作者：
Tsutsumi, Yutaka
中科院分区：
文献类型：
--
作者：
Shimomura, Ryoichi;Tsutsumi, Yutaka
细菌感染的组织学诊断仍然是一个技术挑战。组织化学的应用为提高诊断质量提供了前景。耐甲氧西林金黄色葡萄球菌（MRSA）是最常见的耐药菌，在临床病理诊断中具有重要意义。最近，除了传统的医院获得性MRSA外，社区获得性MRSA也有所扩大。MRSA的免疫组织化学鉴定需要针对种特异性抗原和青霉素结合蛋白2'（PBP2'）的抗体，而种特异性基因和编码PBP2'的mec A可以是原位杂交（ISH）检测的靶标。由于S.金黄色葡萄球菌通常在细胞壁中表达蛋白A，其免疫球蛋白结合能力通过常规制备的切片的加热预处理来恢复。MRSA的ISH技术需要对常规石蜡包埋切片和信号增强序列进行细致的预处理。这篇综述的重点是这种组织病理学的方法，这应该有深刻的潜力，有助于减少和预防MRSA的医院感染。(C)2007爱思唯尔公司All rights reserved.
Histopathological diagnosis of bacterial infection remains a technical challenge. Application of histochemistry provides a prospect of the improvement of diagnostic quality. Methicillin-resistant Staphylococcus aureus (MRSA), the most common drug-resistant bacterium, is of clinical importance in making appropriate histopathological diagnosis. Recently, community-acquired MRSA has expanded, in addition to conventional hospital-acquired MRSA. Immunohistochemical identification of MRSA requires antibodies against both species-specific antigens and penicillin-binding protein 2' (PBP2'), whereas a species-specific gene and mec A encoding PBP2' can be the target of in situ hybridization (ISH) detection. Specificity verification in histochemistry should be emphasized, since S. aureus commonly expresses protein A in the cell wall, whose immunoglobulin-binding capacity is retrieved by heating pretreatment of routinely prepared sections. The ISH technique for MRSA needs meticulous pretreatment of routine paraffin-embedded sections and signal enhancement sequence. This review focuses on such histopathological approaches, which should have profound potential for contributing to decreasing and preventing nosocomial infection of MRSA. (C) 2007 Elsevier Inc. All rights reserved.