A simplified procedure for the purification of trichosanthin (a type 1 ribosome inactivating protein) from Trichosanthes kirilowii root tubers
A simplified procedure for the purification of trichosanthin (a type 1 ribosome inactivating protein) from Trichosanthes kirilowii root tubers
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DOI：
10.1006/prep.1996.0020
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发表时间：
1996-03-01
影响因子：
1.6
通讯作者：
Dandekar, AM
中科院分区：
文献类型：
--
作者：
Bhatia, N;McDonald, KA;Dandekar, AM
采用阳离子交换灌注层析法，建立了一种一步快速、简便的提纯天花粉蛋白的方法。天花粉蛋白是一种1型核糖体失活蛋白。蛋白质的同源性已被其大小、免疫反应性和序列信息所证实。用一次10分钟的层析步骤，可在1天内提纯克量的天花粉蛋白，从而获得0.16%的电泳法纯化天花粉蛋白干块茎。(C)1996年学术出版社。
A one step rapid and simple purification procedure for trichosanthin, a type 1 ribosome inactivating protein, from root tubers of Trichosanthes kirilowii has been developed using cation-exchange perfusion chromatography. The identity of the protein has been confirmed by its size, immunoreactivity, and sequence information. Yields of 0.16% of electrophoretically pure trichosanthin hom dried root tuber have been achieved with a single 10-min chromatographic step giving the ability to purify gram quantities of trichosanthin in 1 day. (C) 1996 Academic Press, Inc.