A new microsporidian parasite, Potaspora morhaphis n. gen., n. sp (Microsporidia) infecting the Teleostean fish, Potamorhaphis guianensis from the River Amazon.: Morphological, ultrastructural and molecular characterization
A new microsporidian parasite, Potaspora morhaphis n. gen., n. sp (Microsporidia) infecting the Teleostean fish, Potamorhaphis guianensis from the River Amazon.: Morphological, ultrastructural and molecular characterization
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DOI：
10.1017/s0031182008004654
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发表时间：
2008-08-01
期刊：
影响因子：
2.4
通讯作者：
Azevedo, C.
中科院分区：
文献类型：
--
作者：
Casal, G.;Matos, E.;Azevedo, C.
根据光学显微镜和超微结构特征，描述了一种寄生于亚马逊河下游淡水鱼贵安鱼体腔壁上的侵染鱼类的微孢子虫。微孢子虫形成白色异种瘤，以大量丝状和吻合的微绒毛为特征。异种瘤在几个发育阶段被完全填充。在所有这些阶段，细胞核都是单核的，并与宿主细胞质直接接触。分生疟原虫通过二元分裂分裂，孢子体起源的非孢子母梨形孢子大小为2-8 +/- 0.3 x 1.5 +/- 0.2 μ m。成熟孢子的极丝在2层中排列成9-10圈。极质体在柄周围有2个明显的区域，并观察到一个电子密集的球体。对小亚基、基因间空间和部分大亚基rRNA基因进行了测序，最大简约性分析将本文描述的微孢子虫置于包括kabatana属、Microgemma属、Spraguea属和Tetramicra属的进化枝中。异种瘤的超微结构形态、孢子发育阶段以及系统发育分析表明，该异种瘤形成了一个新的属和种。
A fish-infecting Microsporidia Potaspora miorhaphis n. gen., n. sp. found adherent to the wall of the coelomic cavity of the freshwater fish, Potamorhaphis guianensis, from lower Amazon River is described, based on light microscope and Ultrastructural characteristics. This microsporidian forms whitish xenomas distinguished by the numerous filiform and anastomosed microvilli. The xenoma was completely filled by several developmental stages. In all of these stages, the nuclei are monokaryotic and develop in direct contact with host cell cytoplasm. The merogonial plasmodium divides by binary fission and the disporoblastic pyriform spores of sporont origin measure 2-8 +/- 0.3 x 1.5 +/- 0.2 mu m. In mature spores the polar filament was arranged into 9-10 coils in 2 layers. The polaroplast had 2 distinct regions around the manubrium and an electron-dense globule was observed. The small Subunit, intergenic space and partial large Subunit rRNA gene were sequenced and maximum parsimony analysis placed the microsporidian described here in the clade that includes the genera kabatana, Microgemma, Spraguea and Tetramicra. The ultrastructural morphology of the xenoma, and the developmental stages including the spores of this microsporidian parasite, as well as the phylogenetic analysis, Suggest the erection of a new genus and species.