Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis
Dramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis
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DOI：
10.1023/a:1016696104185
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发表时间：
2001-08-01
影响因子：
3
通讯作者：
Kim, DS
中科院分区：
文献类型：
--
作者：
Nam, YK;Noh, JK;Kim, DS
转基因泥鳅（Misgurnusmizolepis），其中整个转基因来源于相同的物种，已经通过显微注射泥鳅生长激素（mlGH）基因与泥鳅β-肌动蛋白启动子融合产生.在4，100个注射的卵子中，7.5%来自注射卵子的鱼显示出显着的加速生长，最多比非转基因兄弟姐妹快35倍。许多快速生长的转基因个体表现出非凡的生长习性：它们的体重和总长度（最大鱼达到413 g和41.5 cm）甚至比12岁的正常亲鱼（最大尺寸达到89 g和28 cm）更大和更长。46转基因创始人测试，30个人的转基因传递到下一代的生殖系传播频率范围从2%到33%。后续世代（F-1）的生长性能也显著加速高达35倍，尽管增强生长的水平在转基因品系之间是可变的。建立了三个转基因生殖系，直到F-4，显示出预期的孟德尔遗传的转基因。RT-PCR检测GH mRNA在多种组织中的表达。在这些转基因品系中达到可销售的大小（10 g）所需的时间仅为受精后30-50天，而在非转基因鱼中至少6个月。除了生长增强，还观察到显著提高的饲料转化效率高达1.9倍。
Transgenic mud loaches (Misgurnus mizolepis), in which the entire transgene originated from the same species, have been generated by microinjecting the mud loach growth hormone (mlGH) gene fused to the mud loach beta -actin promoter. Out of 4,100 eggs injected, 7.5% fish derived from the injected eggs showed dramatically accelerated growth, with a maximum of 35-fold faster growth than their non-transgenic siblings. Many fast-growing transgenic individuals showed extraordinary gigantism: their body weight and total length (largest fish attained to 413 g and 41.5 cm) were larger and longer than even those of 12-year-old normal broodstock (maximum size reached to 89 g and 28 cm). Of 46 transgenic founders tested, 30 individuals transmitted the transgene to next generation with a wide range of germ-line transmission frequencies ranging from 2% to 33%. The growth performance of the subsequent generation (F-1) was also dramatically accelerated up to 35-fold, although the levels of enhanced growth were variable among transgenic lines. Three transgenic germ-lines up to F-4 were established, showing the expected Mendelian inheritance of the transgene. Expression of GH mRNA in many tissues was detected by RT-PCR analyses. The time required to attain marketable size (10 g) in these transgenic lines was only 30-50 days after fertilization, while at least 6 months in non-transgenic fish. Besides growth enhancement, significantly improved feed-conversion efficiency up to 1.9-fold was also observed.