Cytological, genetic, and molecular analysis to characterize compatible and incompatible interactions between Medicago truncatula and Colletotrichum trifolii.
Cytological, genetic, and molecular analysis to characterize compatible and incompatible interactions between Medicago truncatula and Colletotrichum trifolii.
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DOI：
10.1094/mpmi.2004.17.8.909
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发表时间：
2004-08
期刊：
影响因子：
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通讯作者：
C. Torregrosa;S. Cluzet;J. Fournier;T. Huguet;P. Gamas;J. Prosperi;M. Esquerré-Tugayé;B. Dumas;C. Jacquet
中科院分区：
文献类型：
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作者：
C. Torregrosa;S. Cluzet;J. Fournier;T. Huguet;P. Gamas;J. Prosperi;M. Esquerré-Tugayé;B. Dumas;C. Jacquet
本研究以模式植物蒺藜苜蓿和苜蓿炭疽病病原菌三叶刺盘孢为材料，建立了一个新的致病系统。筛选几个M。蒺藜品系对车轴草炭疽菌1号生理小种的抗性鉴定结果表明，Jemalong和F83005.5对车轴草炭疽菌1号生理小种的抗性鉴定结果表明，F83005.5对车轴草炭疽菌1号生理小种的抗性鉴定结果表明，症状分析和细胞学研究表明，杰玛龙的抗性与植株的过敏反应有关。将两个选择系杂交，接种C.在所得F1和F2后代上进行三叶草杂交。病害表型分析表明，抗性为显性，可能由一个主效抗性基因控制。通过宏阵列实验分析了抗性的分子组成。对Jemalong和F83005.5株系中92个基因的126个表达序列标签的表达谱进行了比较。上调基因的数量与抗性表型之间存在很强的相关性。在接种后48 h出现了较大的差异，超过40%的测试基因在Jemalong线相比，只有10%的敏感线上调。有趣的是，在接种C.三叶草。
In this study, a new pathosystem was established using the model plant Medicago truncatula and Colletotrichum trifolii, the causal agent of anthracnose on Medicago sativa. Screening of a few M. truncatula lines identified Jemalong and F83005.5 as resistant and susceptible to Colletotrichum trifolii race 1, respectively. Symptom analysis and cytological studies indicated that resistance of Jemalong was associated with a hypersensitive response of the plant. The two selected lines were crossed, and inoculations with C. trifolii were performed on the resulting F1 and F2 progenies. Examination of the disease phenotypes indicated that resistance was dominant and was probably due to a major resistance gene. Molecular components of the resistance were analyzed through macroarray experiments. Expression profiling of 126 expressed sequence tags corresponding to 92 genes, which were selected for their putative functions in plant defense or signal transduction, were compared in Jemalong and F83005.5 lines. A strong correlation was observed between the number of up-regulated genes and the resistance phenotype. Large differences appeared at 48 h postinoculation; more than 40% of the tested genes were up-regulated in the Jemalong line compared with only 10% in the susceptible line. Interestingly, some nodulin genes were also induced in the resistant line upon inoculation with C. trifolii.