Genetic locus encoding functions involved in biosynthesis and outer membrane localization of xanthomonadin in Xanthomonas oryzae pv. oryzae
Genetic locus encoding functions involved in biosynthesis and outer membrane localization of xanthomonadin in Xanthomonas oryzae pv. oryzae
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DOI：
10.1128/jb.184.13.3539-3548.2002
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发表时间：
2002-07-01
影响因子：
3.2
通讯作者：
Sonti, RV
中科院分区：
文献类型：
--
作者：
Goel, AK;Rajagopal, L;Sonti, RV
Xanthomonadins是黄单胞菌属特有的膜结合、溴化、芳基多烯类色素。我们鉴定了水稻白叶枯病菌的一个遗传基因座(PIG)。含有四个开放阅读框(ORF)，是生产黄单胞菌素所必需的。其中三个ORF与酰基载体蛋白、脱水酶和酰基转移酶同源，提示黄单胞菌素的生物合成途径为II型聚酮合成酶。第四个ORF在数据库中没有同源基因。本研究首次报道了水稻白叶枯病菌的外膜定位需要一个编码在猪基因组中的细胞质膜蛋白。米饭。我们还报告了一个新的145个碱基的回文黄单胞菌重复基因间共识元件的鉴定，该元件存在于猪基因座的两个地方。我们估计，在稻瘟病菌的基因组中可能存在超过100个拷贝的该元件。水稻和其他黄单胞菌。
Xanthomonadins are membrane-bound, brominated, aryl-polyene pigments specific to the genus Xanthomonas. We have characterized a genetic locus (pig) from Xanthomonas oryzae pv. oryzae which contains four open reading frames (ORFs) that are essential for xanthomonadin production. Three of these ORFs are homologous to acyl carrier proteins, dehydratases, and acyl transferases, suggesting a type II polyketide synthase pathway for xanthomonadin biosynthesis. The fourth ORF has no homologue in the database. For the first time, we report that a putative cytoplasmic membrane protein encoded in the pig locus is required for outer membrane localization of xanthomonadin in X. oryzae pv. oryzae. We also report the identification of a novel 145-bp palindromic Xanthomonas repetitive intergenic consensus element that is present in two places in the pig locus. We estimate that more than 100 copies of this element might be present in the genome of X. oryzae pv. oryzae and other xanthomonads.