Quantum dots as fluorescent labels for quantitative detection of Salmonella typhimurium in chicken carcass wash water.
Quantum dots as fluorescent labels for quantitative detection of Salmonella typhimurium in chicken carcass wash water.
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DOI：
10.4315/0362-028x-68.6.1241
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发表时间：
2005-06
影响因子：
2
通讯作者：
Liju Yang;Yanbin Li
中科院分区：
文献类型：
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作者：
Liju Yang;Yanbin Li
荧光半导体量子点是近年来出现的一类新型的、有前途的生物检测荧光标记物。在这项研究中，量子点作为荧光标记的免疫定量检测食源性致病菌。使用抗沙门氏菌抗体包被的磁珠从鸡屠体洗涤水中分离鼠伤寒沙门氏菌细胞，并与生物素标记的抗沙门氏菌二抗反应。加入用链霉亲和素包被的量子点以与二抗上的生物素反应。测量量子点产生的荧光强度为微生物检测提供了一种定量方法。在10（3）~ 10（7）CFU/ml范围内，鸡屠体清洗水中鼠伤寒沙门氏菌的细胞数（log N）与荧光强度（FI）呈线性关系。回归模型可表示为FI = 198.6 Log N - 639.03，R2 = 0.96。该方法的检出限为10（3）CFU/ml。
Fluorescent semiconductor quantum dots have recently emerged as a novel and promising class of fluorescent labels for biological detection. In this study, quantum dots were used as fluorescent labels in immunoassays for quantitative detection of foodborne pathogenic bacteria. Salmonella Typhimurium cells were separated from chicken carcass wash water using anti-Salmonella antibody coated magnetic beads and reacted to secondary biotin-labeled anti-Salmonella antibody. Quantum dots coated with streptavidin were added to react with biotin on the secondary antibody. Measurement of the intensity of fluorescence produced by quantum dots provided a quantitative method for microbial detection. A linear relationship between Salmonella Typhimurium cell number (log N) in the samples of chicken carcass wash water and the fluorescence intensity (FI) was found for the cell numbers ranging from 10(3) to 10(7) CFU/ml. The regression model can be expressed as FI = 198.6 Log N - 639.03 with R2 = 0.96. The detection limit of this method was 10(3) CFU/ml.