Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.
Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting α-Actinin 4 binding to actin filaments.
复制标题
聚 L-鸟氨酸通过促进 α-肌动蛋白 4 与肌动蛋白丝的结合来增强神经干/祖细胞的迁移。
DOI：
10.1038/srep37681
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发表时间：
2016-11-22
影响因子：
4.6
通讯作者：
Hu R
中科院分区：
文献类型：
--
作者：
Ge H;Yu A;Chen J;Yuan J;Yin Y;Duanmu W;Tan L;Yang Y;Lan C;Chen W;Feng H;Hu R
募集神经干/祖细胞（NSPCs）用于脑损伤后的修复是一种有前途的再生治疗策略。该策略涉及促进NSPCs的增殖、迁移和神经元分化。迄今为止，缺乏促进这些过程以增强神经再生的生物材料是细胞替代疗法的障碍。我们的前期研究表明，与多聚赖氨酸（PLL）和纤维连接蛋白（FN）相比，多聚L-鸟氨酸（PO）培养的NSPCs增殖更活跃，分化为更多的神经元。本研究证明PO能促进体外培养的NSPCs迁移，其机制是PO激活了首次被证实在NSPCs中表达的α-Actinins 4（ACTN 4），促进丝状伪足的形成，从而促进NSPCs迁移。总而言之，与PLL和FN相比，PO可能是再生治疗策略中移植生物材料的更好候选材料。
The recruitment of neural stem/progenitor cells (NSPCs) for brain restoration after injury is a promising regenerative therapeutic strategy. This strategy involves enhancing proliferation, migration and neuronal differentation of NSPCs. To date, the lack of biomaterials, which facilitate these processes to enhance neural regeneration, is an obstacle for the cell replacement therapies. Our previous study has shown that NSPCs grown on poly-L-ornithine (PO) could proliferate more vigorously and differentiate into more neurons than that on Poly-L-Lysine (PLL) and Fibronectin (FN). Here, we demonstrate that PO could promote migration of NSPCs in vitro, and the underlying mechanism is PO activates α-Actinins 4 (ACTN4), which is firstly certified to be expessed in NSPCs, to promote filopodia formation and therefore enhances NSPCs migration. Taken together, PO might serve as a better candidate for transplanted biomaterials in the regenerative therapeutic strategy, compared with PLL and FN.
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